Functional transcriptomic annotation and protein–protein interaction analysis identify EZH2 and UBE2C as key upregulated proteins in ovarian cancer

Sandra Martínez-Canales, Miguel López de Rodas, Miriam Nuncia-Cantarero, Raquel Páez, Eitan Amir, Balázs Győrffy, Atanasio Pandiella, Eva María Galán-Moya, Alberto Ocaña

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6 Citations (Scopus)

Abstract

Although early stage ovarian cancer is in most cases a curable disease, some patients relapse even with appropriate adjuvant treatment. Therefore, the identification of patient and tumor characteristics to better stratify risk and guide rational drug development is desirable. Using transcriptomic functional annotation followed by protein–protein interacting (PPI) network analyses, we identified functions that were upregulated and associated with detrimental outcome in patients with early stage ovarian cancer. Some of the identified functions included cell cycle, cell division, signal transduction/protein modification, cellular response to extracellular stimuli or transcription regulation, among others. Genes within these functions included AURKA, AURKB, CDK1, BIRC5, or CHEK1 among others. Of note, the histone-lysine N-methyltransferase (EZH2) and the ubiquitin-conjugating enzyme E2C (UBE2C) genes were found to be upregulated and amplified in 10% and 6% of tumors, respectively. Of note, EZH2 and UBE2C were identified as principal interacting proteins of druggable networks. In conclusion, we describe a set of genes overexpressed in ovarian cancer with potential for therapeutic intervention including EZH2 and UBE2C.

Original languageEnglish
Pages (from-to)1896-1907
Number of pages12
JournalCancer Medicine
Volume7
Issue number5
DOIs
Publication statusPublished - May 2018

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Keywords

  • Clinical outcome
  • EZH2
  • Ovarian cancer
  • UBE2C
  • druggable proteins
  • protein–protein interaction

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Martínez-Canales, S., López de Rodas, M., Nuncia-Cantarero, M., Páez, R., Amir, E., Győrffy, B., Pandiella, A., Galán-Moya, E. M., & Ocaña, A. (2018). Functional transcriptomic annotation and protein–protein interaction analysis identify EZH2 and UBE2C as key upregulated proteins in ovarian cancer. Cancer Medicine, 7(5), 1896-1907. https://doi.org/10.1002/cam4.1406