Functional Thrombin Receptors on Human T Lymphoblastoid Cells

A. Tordai, John W. Fenton, Thomas Andersen, Erwin W. Gelfand

Research output: Contribution to journalArticle

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Abstract

Human α-thrombin stimulated five different T lymphoblastoid cell lines to increase intracellular free Ca2+ concentrations, whereas five B cell lines did not similarly respond. The T cell intracellular free Ca2+ increased rapidly, plateaued within 10 to 20 s, and then declined without any measurable sustained intracellular free Ca2+ elevation. Liberation of inositol trisphosphate peaked within 60 s, and a rapid and sustained activation of protein kinase C was induced. The thrombin-specific inhibitor, hirudin, completely blocked the response to α-thrombin. Catalytically inactivated forms of α-thrombin failed to stimulate the T cells, whereas trypsin, γ-thrombin (which lacks fibrinogen clotting activity), or the synthetic 14-amino acid thrombin receptor-activation peptide stimulated T cells, but required ∼10-, ∼15-, or ∼100-fold higher concentrations, respectively, when compared to α-thrombin. Stimulation by thrombin receptor-activation peptide was desensitized by α-thrombin, and vice versa, but α-thrombin did not desensitize stimulation by mAb to the TCR/CD3 Ag. The presence of the receptor on T but not on B cells was confirmed by flow-cytometric analysis using a mAb against the human thrombin receptor. These findings demonstrate functional thrombin receptors on T lymphoblastoid cells that may be capable of activating the cells, independently of an ongoing immune response.

Original languageEnglish
Pages (from-to)4876-4886
Number of pages11
JournalJournal of Immunology
Volume150
Issue number11
Publication statusPublished - Jun 1 1993

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Thrombin Receptors
Thrombin
T-Lymphocytes
B-Lymphocytes
Amino Acid Receptors
Peptide T
Hirudins
Cell Line
Inositol
Trypsin
Fibrinogen
Protein Kinase C
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Tordai, A., Fenton, J. W., Andersen, T., & Gelfand, E. W. (1993). Functional Thrombin Receptors on Human T Lymphoblastoid Cells. Journal of Immunology, 150(11), 4876-4886.

Functional Thrombin Receptors on Human T Lymphoblastoid Cells. / Tordai, A.; Fenton, John W.; Andersen, Thomas; Gelfand, Erwin W.

In: Journal of Immunology, Vol. 150, No. 11, 01.06.1993, p. 4876-4886.

Research output: Contribution to journalArticle

Tordai, A, Fenton, JW, Andersen, T & Gelfand, EW 1993, 'Functional Thrombin Receptors on Human T Lymphoblastoid Cells', Journal of Immunology, vol. 150, no. 11, pp. 4876-4886.
Tordai A, Fenton JW, Andersen T, Gelfand EW. Functional Thrombin Receptors on Human T Lymphoblastoid Cells. Journal of Immunology. 1993 Jun 1;150(11):4876-4886.
Tordai, A. ; Fenton, John W. ; Andersen, Thomas ; Gelfand, Erwin W. / Functional Thrombin Receptors on Human T Lymphoblastoid Cells. In: Journal of Immunology. 1993 ; Vol. 150, No. 11. pp. 4876-4886.
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