Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana

Balázs Fehér, L. Kozma-Bognár, E. Kévei, Anita Hajdu, Melanie Binkert, Seth Jon Davis, Eberhard Schäfer, Roman Ulm, Ferenc Nagy

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Circadian clocks regulate many molecular and physiological processes in Arabidopsis (Arabidopsis thaliana), allowing the timing of these processes to occur at the most appropriate time of the day in a 24-h period. The accuracy of timing relies on the synchrony of the clock and the environmental day/night cycle. Visible light is the most potent signal for such synchronization, but light-induced responses are also rhythmically attenuated (gated) by the clock. Here, we report a similar mutual interaction of the circadian clock and non-damaging photomorphogenic UV-B light. We show that low-intensity UV-B radiation acts as entraining signal for the clock. UV RESISTANCE LOCUS 8 (UVR8) and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are required, but ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) are dispensable for this process. UV-B responsiveness of clock gene expression suggests that photomorphogenic UV-B entrains the plant clock through transcriptional activation. We also demonstrate that UV-B induction of gene expression under these conditions is gated by the clock in a HY5/HYH-independent manner. The arrhythmic early flowering 3-4 mutant showed non-gated, high-level gene induction by UV-B, yet displayed no increased tolerance to UV-B stress. Thus, the temporal restriction of UV-B responsiveness by the circadian clock can be considered as saving resources during acclimation without losing fitness.

Original languageEnglish
Pages (from-to)37-48
Number of pages12
JournalPlant Journal
Volume67
Issue number1
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Circadian Clocks
Arabidopsis
circadian rhythm
ultraviolet radiation
Arabidopsis thaliana
Physiological Phenomena
Gene Expression
Light
Acclimatization
Ultraviolet Rays
Transcriptional Activation
Radiation
gene expression
Genes
gene induction
transcriptional activation
acclimation
flowering
mutants
loci

Keywords

  • circadian clock
  • circadian gating
  • entrainment
  • gene expression
  • ultraviolet-B light
  • UV-B tolerance

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana. / Fehér, Balázs; Kozma-Bognár, L.; Kévei, E.; Hajdu, Anita; Binkert, Melanie; Davis, Seth Jon; Schäfer, Eberhard; Ulm, Roman; Nagy, Ferenc.

In: Plant Journal, Vol. 67, No. 1, 07.2011, p. 37-48.

Research output: Contribution to journalArticle

Fehér, Balázs ; Kozma-Bognár, L. ; Kévei, E. ; Hajdu, Anita ; Binkert, Melanie ; Davis, Seth Jon ; Schäfer, Eberhard ; Ulm, Roman ; Nagy, Ferenc. / Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana. In: Plant Journal. 2011 ; Vol. 67, No. 1. pp. 37-48.
@article{3c77ca52462d4029a082890cd9e05f7e,
title = "Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana",
abstract = "Circadian clocks regulate many molecular and physiological processes in Arabidopsis (Arabidopsis thaliana), allowing the timing of these processes to occur at the most appropriate time of the day in a 24-h period. The accuracy of timing relies on the synchrony of the clock and the environmental day/night cycle. Visible light is the most potent signal for such synchronization, but light-induced responses are also rhythmically attenuated (gated) by the clock. Here, we report a similar mutual interaction of the circadian clock and non-damaging photomorphogenic UV-B light. We show that low-intensity UV-B radiation acts as entraining signal for the clock. UV RESISTANCE LOCUS 8 (UVR8) and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are required, but ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) are dispensable for this process. UV-B responsiveness of clock gene expression suggests that photomorphogenic UV-B entrains the plant clock through transcriptional activation. We also demonstrate that UV-B induction of gene expression under these conditions is gated by the clock in a HY5/HYH-independent manner. The arrhythmic early flowering 3-4 mutant showed non-gated, high-level gene induction by UV-B, yet displayed no increased tolerance to UV-B stress. Thus, the temporal restriction of UV-B responsiveness by the circadian clock can be considered as saving resources during acclimation without losing fitness.",
keywords = "circadian clock, circadian gating, entrainment, gene expression, ultraviolet-B light, UV-B tolerance",
author = "Bal{\'a}zs Feh{\'e}r and L. Kozma-Bogn{\'a}r and E. K{\'e}vei and Anita Hajdu and Melanie Binkert and Davis, {Seth Jon} and Eberhard Sch{\"a}fer and Roman Ulm and Ferenc Nagy",
year = "2011",
month = "7",
doi = "10.1111/j.1365-313X.2011.04573.x",
language = "English",
volume = "67",
pages = "37--48",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Functional interaction of the circadian clock and UV RESISTANCE LOCUS 8-controlled UV-B signaling pathways in Arabidopsis thaliana

AU - Fehér, Balázs

AU - Kozma-Bognár, L.

AU - Kévei, E.

AU - Hajdu, Anita

AU - Binkert, Melanie

AU - Davis, Seth Jon

AU - Schäfer, Eberhard

AU - Ulm, Roman

AU - Nagy, Ferenc

PY - 2011/7

Y1 - 2011/7

N2 - Circadian clocks regulate many molecular and physiological processes in Arabidopsis (Arabidopsis thaliana), allowing the timing of these processes to occur at the most appropriate time of the day in a 24-h period. The accuracy of timing relies on the synchrony of the clock and the environmental day/night cycle. Visible light is the most potent signal for such synchronization, but light-induced responses are also rhythmically attenuated (gated) by the clock. Here, we report a similar mutual interaction of the circadian clock and non-damaging photomorphogenic UV-B light. We show that low-intensity UV-B radiation acts as entraining signal for the clock. UV RESISTANCE LOCUS 8 (UVR8) and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are required, but ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) are dispensable for this process. UV-B responsiveness of clock gene expression suggests that photomorphogenic UV-B entrains the plant clock through transcriptional activation. We also demonstrate that UV-B induction of gene expression under these conditions is gated by the clock in a HY5/HYH-independent manner. The arrhythmic early flowering 3-4 mutant showed non-gated, high-level gene induction by UV-B, yet displayed no increased tolerance to UV-B stress. Thus, the temporal restriction of UV-B responsiveness by the circadian clock can be considered as saving resources during acclimation without losing fitness.

AB - Circadian clocks regulate many molecular and physiological processes in Arabidopsis (Arabidopsis thaliana), allowing the timing of these processes to occur at the most appropriate time of the day in a 24-h period. The accuracy of timing relies on the synchrony of the clock and the environmental day/night cycle. Visible light is the most potent signal for such synchronization, but light-induced responses are also rhythmically attenuated (gated) by the clock. Here, we report a similar mutual interaction of the circadian clock and non-damaging photomorphogenic UV-B light. We show that low-intensity UV-B radiation acts as entraining signal for the clock. UV RESISTANCE LOCUS 8 (UVR8) and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are required, but ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) are dispensable for this process. UV-B responsiveness of clock gene expression suggests that photomorphogenic UV-B entrains the plant clock through transcriptional activation. We also demonstrate that UV-B induction of gene expression under these conditions is gated by the clock in a HY5/HYH-independent manner. The arrhythmic early flowering 3-4 mutant showed non-gated, high-level gene induction by UV-B, yet displayed no increased tolerance to UV-B stress. Thus, the temporal restriction of UV-B responsiveness by the circadian clock can be considered as saving resources during acclimation without losing fitness.

KW - circadian clock

KW - circadian gating

KW - entrainment

KW - gene expression

KW - ultraviolet-B light

KW - UV-B tolerance

UR - http://www.scopus.com/inward/record.url?scp=79959747572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959747572&partnerID=8YFLogxK

U2 - 10.1111/j.1365-313X.2011.04573.x

DO - 10.1111/j.1365-313X.2011.04573.x

M3 - Article

C2 - 21395889

AN - SCOPUS:79959747572

VL - 67

SP - 37

EP - 48

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 1

ER -