Functional genomics of calcium channels in human melanoma cells

Tamás Deli, Norbert Varga, Attila Ádám, István Kenessey, Erzsébet Rásó, László G. Puskás, József Tóvári, János Fodor, Mónika Fehér, Gyula P. Szigeti, László Csernoch, József Tímár

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46 Citations (Scopus)

Abstract

Ca2+-signaling of human melanoma is in the focus of intensive research since the identification of the role of WNT-signaling in melanomagenesis. Genomic and functional studies pointed to the important role of various Ca2+ channels in melanoma, but these data were contradictory. In the present study we clearly demonstrate, in a number of different ways including microarray analysis, DNA sequencing and immunocytochemistry, that various human melanoma cell lines and melanoma tissues overexpress ryanodine receptor type 2 (RyR2) and express P2X7 channel proteins as compared to melanocytes. These channels, although retain some of their usual characteristics and pharmacological properties, display unique features in melanoma cells, including a functional interaction between the two molecules. Unlike P2X7, RyR2 does not function as a calcium channel. On the other hand, the P2X7 receptor has an antiapoptotic function in melanoma cells, since ATP-activation suppresses induced apoptosis, while knock down of the gene expression significantly enhances that.

Original languageEnglish
Pages (from-to)55-65
Number of pages11
JournalInternational Journal of Cancer
Volume121
Issue number1
DOIs
Publication statusPublished - Jul 1 2007

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Keywords

  • Apoptosis
  • Ca transient
  • Human melanoma
  • P2X receptor
  • Ryanodine receptor-2

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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