Functional expression of a multidrug P-glycoprotein transporter of Leishmania

Fernando Cortés-Selva, Francisco Muñoz-Martínez, Attila Iliás, Antonio Ignacio Jiménez, András Váradi, Francisco Gamarro, Santiago Castanys

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

P-glycoprotein (Pgp) transporters play an important role in multidrug resistance in eukaryotic cells and in protozoan parasites such as Leishmania. To search for new reversal agents of the Leishmania tropica Pgp, we developed a screening assay using the Baculovirus-insect cell expression system. We demonstrated a MgATP-dependent, vanadate-sensitive transport of Hoechst 33342 in membrane preparations of Sf9 insect cells expressing Pgp. We have found that dihydro-β-agarofuran sesquiterpenes from Maytenus cuzcoina inhibited Hoechst 33342 transport that correlates with their reversal effect in a multidrug-resistant L. tropica line overexpressing Pgp. The results suggest that Sf9 cell membrane Hoechst 33342 transport system represents an efficient tool for examining the interactions of Leishmania Pgp with pharmacological agents.

Original languageEnglish
Pages (from-to)502-507
Number of pages6
JournalBiochemical and biophysical research communications
Volume329
Issue number2
DOIs
Publication statusPublished - Apr 8 2005

Keywords

  • ABC transporter
  • Drug transport
  • Insect cells
  • Leishmania
  • Modulator
  • Multidrug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Cortés-Selva, F., Muñoz-Martínez, F., Iliás, A., Jiménez, A. I., Váradi, A., Gamarro, F., & Castanys, S. (2005). Functional expression of a multidrug P-glycoprotein transporter of Leishmania. Biochemical and biophysical research communications, 329(2), 502-507. https://doi.org/10.1016/j.bbrc.2005.02.009