Functional effects of domain deletions in a multidomain serine protease, C1r

Sándor Cseh, Péter Gál, Miklós Sárvári, József Dobó, Zsolt Lorincz, Verne N. Schumaker, Péter Závodszky

Research output: Contribution to journalArticle

14 Citations (Scopus)


The C1r subcomponent of the first component of complement is a complex, multidomain glycoprotein containing five regulatory or binding modules in addition to the serine protease domain. To reveal the functional role of the N-terminal regulatory domains, two deletion mutants of C1r were constructed. One mutant comprises the N-terminal half of domain I joined to the second half of the highly homologous domain III, resulting in one chimeric domain in the N-terminal region, instead of domains I-III. In the second mutant most of the N-terminal portion of domain I was deleted. Both deletion mutants were expressed in the baculovirus-insect cell expression system with yields typical of wild type C1r. Both mutants maintained the ability of the wild type C1r to dimerize. The folding and secretion of the recombinant proteins was not affected by these deletions, and Cl-inhibitor binding was not impaired. The stability of the zymogen was significantly decreased however, indicating that the N-terminal region of the C1r molecule contains essential elements involved in the control of activation of the serine protease module. Tetramer formation with C1s in the presence of Ca2+ was abolished by both deletions. We suggest that the first domain of C1r is essential for tetramer formation, since the deletion of domain I from C1r impairs this interaction.

Original languageEnglish
Pages (from-to)351-359
Number of pages9
JournalMolecular Immunology
Issue number4-5
Publication statusPublished - Jan 1 1996


  • Complement activation
  • baculovirus
  • glycoprotein
  • zymogen

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Functional effects of domain deletions in a multidomain serine protease, C1r'. Together they form a unique fingerprint.

  • Cite this