Functional diversity of potato SNF1-related kinases tested in Saccharomyces cerevisiae

Ágnes Lovas, Anita Sós-Hegedus, Andrea Bimbó, Z. Bánfalvi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sucrose nonfermenting 1 catalytic subunit (SNF1)-type protein kinases are members of a metabolite-sensing protein kinase family distributed ubiquitously from yeast to plants and animals. In yeast cells, SNF1 acts in complex with the activator subunit SNF4 and a member of the SIP1/SIP2/GAL83 family responsible for substrate definition. The potato (Solanum tuberosum) genome possesses at least two SnRK1s, designated PKIN1 and StubSNF1. In this study, potato kinase 1 (PKIN1) and StubSNF1 were analysed in the yeast two-hybrid system and characterised by suppression of yeast mutations. It was shown that StubSNF1 interacted with the GAL83 ortholog of potato, StubGAL83, and complemented the Δsnf1 mutation. Moreover, it suppressed Δsnf4 and Δsip1,Δsip2,Δgal83 deficiencies. In contrast, PKIN1 was unable to interact with StubGAL83 and did not rescue the yeast mutants. These data suggest different functions for PKIN1 and StubSNF1 in potato.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalGene
Volume321
Issue number1-2
DOIs
Publication statusPublished - Dec 4 2003

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Solanum tuberosum
Saccharomyces cerevisiae
Sucrose
Catalytic Domain
Phosphotransferases
Yeasts
Protein Kinases
Two-Hybrid System Techniques
Mutation
Genome

Keywords

  • Catabolite regulation
  • Invertase
  • PKIN1
  • Protein-protein interaction
  • Serine/threonine protein kinase
  • SnRK

ASJC Scopus subject areas

  • Genetics

Cite this

Functional diversity of potato SNF1-related kinases tested in Saccharomyces cerevisiae. / Lovas, Ágnes; Sós-Hegedus, Anita; Bimbó, Andrea; Bánfalvi, Z.

In: Gene, Vol. 321, No. 1-2, 04.12.2003, p. 123-129.

Research output: Contribution to journalArticle

Lovas, Ágnes ; Sós-Hegedus, Anita ; Bimbó, Andrea ; Bánfalvi, Z. / Functional diversity of potato SNF1-related kinases tested in Saccharomyces cerevisiae. In: Gene. 2003 ; Vol. 321, No. 1-2. pp. 123-129.
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