Functional conservation of CYCLOPS in crack entry legume Arachis hypogaea

Debapriya Rajlakshmi Das, Beatrix Horváth, Anindya Kundu, P. Kaló, Maitrayee DasGupta

Research output: Contribution to journalArticle

Abstract

Root nodule symbiosis in legumes is established following interaction of compatible rhizobia that activates an array of genes, commonly known as symbiotic-pathway, resulting in nodule development. In model legumes, bacterial entry mainly occurs through infection thread involving the expression of transcription factor CYCLOPS/IPD3. Here we show the functional analysis of AhCYCLOPS in Arachis hypogaea where bacteria invade roots through epidermal cracks. Exploiting significant cross-species domain conservation, trans-complementation experiments involving ectopic expression of AhCYCLOPS in transgenic hairy-roots of Medicago truncatula ipd3 mutants resulted in functional complementation of Medicago nodules. Moreover, native promoter of AhCYCLOPS was sufficient for this cross-species complementation irrespective of the different modes of infection of roots by rhizobia and nodule ontology. To unravel the role of AhCYCLOPS during ‘crack-entry’ nodulation in A. hypogaea, RNAi of AhCYCLOPS was performed which resulted in delayed nodule inception followed by drastic reduction in nodule number on transgenic hairy-roots. The infection zone of a significant number of RNAi nodules showed presence of infected cells with enlarged nucleus and rod shaped undifferentiated bacteria. Expression analysis showed downregulation of several nodulation responsible effectors endorsing the compromised condition of RNAi roots. Together, the results indicated that AhCYCLOPS plays an important role in A. hypogaea nodule development.

Original languageEnglish
JournalPlant Science
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Arachis hypogaea
RNA Interference
Fabaceae
Rhizobium
legumes
Infection
Medicago truncatula
Bacteria
Medicago
nodulation
Symbiosis
infection
Transcription Factors
Down-Regulation
bacteria
root nodules
symbiosis
transcription factors
Genes
promoter regions

Keywords

  • Arachis hypogaea
  • CYCLOPS
  • Medicago truncatula
  • Nodulation
  • Symbiotic nitrogen fixation

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Functional conservation of CYCLOPS in crack entry legume Arachis hypogaea. / Das, Debapriya Rajlakshmi; Horváth, Beatrix; Kundu, Anindya; Kaló, P.; DasGupta, Maitrayee.

In: Plant Science, 01.01.2018.

Research output: Contribution to journalArticle

Das, Debapriya Rajlakshmi ; Horváth, Beatrix ; Kundu, Anindya ; Kaló, P. ; DasGupta, Maitrayee. / Functional conservation of CYCLOPS in crack entry legume Arachis hypogaea. In: Plant Science. 2018.
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