Functional characterization of phytochrome interacting factor 3 for the Arabidopsis thaliana circadian clockwork

András Viczián, Stefan Kircher, Erzsébet Fejes, Andrew J. Millar, Eberhard Schäfer, L. Kozma-Bognár, Ferenc Nagy

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Light, in a quality- and quantity-dependent fashion, induces nuclear import of the plant photoreceptors phytochromes and promotes interaction of these receptors with transcription factors including PHYTOCHROME INTERACTING FACTOR 3 (PIF3). PIF3 was shown to form in vitro a ternary complex with the G-box element of the promoters of LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and the Pfr conformer of phytochromes. CCA1 and LHY together with TIMING OF CAB EXPRESSION 1 (TOC1) constitute a transcriptional feed-back loop that is essential for a functional circadian clock in Arabidopsis. These findings led to the hypothesis that the PIF3-containing ternary complex regulates transcription of light-responsive genes and is involved in phototransduction to the central circadian clockwork. Here we report that (i) overexpression or lack of biologically functional PIF3 does not affect period length of rhythmic gene expression or red-light-induced resetting of the circadian clock and (ii) the transcription of PIF3 displays a low-amplitude circadian rhythm. We demonstrated previously that irradiation of etiolated seedlings induces rapid, phytochrome-controlled degradation of PIF3. Here we show that nuclear-localized PIF3 accumulates to relatively high levels by the end of the light phase in seedlings grown under diurnal conditions. Taken together, we show that (i) PIF3 does not play a significant role in controlling light input to and function of the circadian clockwork and (ii) a yet unknown mechanism limits phytochrome-induced degradation of PIF3 at the end of the day under diurnal conditions. JSPP

Original languageEnglish
Pages (from-to)1591-1602
Number of pages12
JournalPlant and Cell Physiology
Volume46
Issue number10
DOIs
Publication statusPublished - Oct 2005

Fingerprint

Phytochrome
phytochrome
Arabidopsis
Arabidopsis thaliana
Circadian Clocks
circadian rhythm
Light
Hypocotyl
Seedlings
hypocotyls
Plant Photoreceptors
transcription (genetics)
cabs (equipment)
Light Signal Transduction
phototransduction
degradation
Cell Nucleus Active Transport
seedlings
light quality
Circadian Rhythm

Keywords

  • Circadian clock
  • Light
  • Phototransduction
  • Phytochrome
  • Protein degradation

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Functional characterization of phytochrome interacting factor 3 for the Arabidopsis thaliana circadian clockwork. / Viczián, András; Kircher, Stefan; Fejes, Erzsébet; Millar, Andrew J.; Schäfer, Eberhard; Kozma-Bognár, L.; Nagy, Ferenc.

In: Plant and Cell Physiology, Vol. 46, No. 10, 10.2005, p. 1591-1602.

Research output: Contribution to journalArticle

Viczián, András ; Kircher, Stefan ; Fejes, Erzsébet ; Millar, Andrew J. ; Schäfer, Eberhard ; Kozma-Bognár, L. ; Nagy, Ferenc. / Functional characterization of phytochrome interacting factor 3 for the Arabidopsis thaliana circadian clockwork. In: Plant and Cell Physiology. 2005 ; Vol. 46, No. 10. pp. 1591-1602.
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