Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize

G. Horváth, M. Droppa, L. Mustárdy, A. Faludi-Dániel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A procedure was developed to isolate mesophyll and bundle sheath chloroplasts of a high degree of intactness and low cross-contamination. Light-induced 14CO2 fixation of isolated chloroplasts was similar to that of protoplasts and cells in that it was low and was stimulated by the addition of exogenous substrates. O2 evolution was absent in both bundle sheath chloroplasts and cells. The flash-induced 515 nm absorbance change of intact mesophyll chloroplasts showed a biphasic rise, previously known to be a characteristic only of intact algae. With bundle sheath chloroplasts or cells, no 515 nm signal could be detected. In the presence of 10 μmol l-1 phenazine methosulphate, bundle sheath chloroplasts exhibited a flash-induced 515 nm signal with a monophasic rise and amplitude comparable to that of the mesophyll chloroplasts. A similar signal was obtained with bundle sheath chloroplasts suspended in an extract prepared from the mesophyll tissue. Both the substrate stimulation of the CO2 fixation and the reconstitution of the 515 nm signal in bundle sheath chloroplasts by the mesophyll extract indicate the requirement of cooperation between the mesophyll and bundle sheath cells of maize leaves.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalPlanta
Volume141
Issue number3
DOIs
Publication statusPublished - Jan 1978

Fingerprint

bundle sheath cells
Protoplasts
Chloroplasts
mesophyll
protoplasts
functional properties
Zea mays
chloroplasts
corn
algae
Methylphenazonium Methosulfate
phenazines
cross contamination
extracts
cells
absorbance
Light

Keywords

  • Bundle sheath
  • Chloroplasts
  • Mesophyll
  • Photosynthetics (C)
  • Protoplasts

ASJC Scopus subject areas

  • Plant Science

Cite this

Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize. / Horváth, G.; Droppa, M.; Mustárdy, L.; Faludi-Dániel, A.

In: Planta, Vol. 141, No. 3, 01.1978, p. 239-244.

Research output: Contribution to journalArticle

@article{9c4117e8fd104a04b5afa5962b2bfab2,
title = "Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize",
abstract = "A procedure was developed to isolate mesophyll and bundle sheath chloroplasts of a high degree of intactness and low cross-contamination. Light-induced 14CO2 fixation of isolated chloroplasts was similar to that of protoplasts and cells in that it was low and was stimulated by the addition of exogenous substrates. O2 evolution was absent in both bundle sheath chloroplasts and cells. The flash-induced 515 nm absorbance change of intact mesophyll chloroplasts showed a biphasic rise, previously known to be a characteristic only of intact algae. With bundle sheath chloroplasts or cells, no 515 nm signal could be detected. In the presence of 10 μmol l-1 phenazine methosulphate, bundle sheath chloroplasts exhibited a flash-induced 515 nm signal with a monophasic rise and amplitude comparable to that of the mesophyll chloroplasts. A similar signal was obtained with bundle sheath chloroplasts suspended in an extract prepared from the mesophyll tissue. Both the substrate stimulation of the CO2 fixation and the reconstitution of the 515 nm signal in bundle sheath chloroplasts by the mesophyll extract indicate the requirement of cooperation between the mesophyll and bundle sheath cells of maize leaves.",
keywords = "Bundle sheath, Chloroplasts, Mesophyll, Photosynthetics (C), Protoplasts",
author = "G. Horv{\'a}th and M. Droppa and L. Must{\'a}rdy and A. Faludi-D{\'a}niel",
year = "1978",
month = "1",
doi = "10.1007/BF00388338",
language = "English",
volume = "141",
pages = "239--244",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Functional characteristics of intact chloroplasts isolated from mesophyll protoplasts and bundle sheath cells of maize

AU - Horváth, G.

AU - Droppa, M.

AU - Mustárdy, L.

AU - Faludi-Dániel, A.

PY - 1978/1

Y1 - 1978/1

N2 - A procedure was developed to isolate mesophyll and bundle sheath chloroplasts of a high degree of intactness and low cross-contamination. Light-induced 14CO2 fixation of isolated chloroplasts was similar to that of protoplasts and cells in that it was low and was stimulated by the addition of exogenous substrates. O2 evolution was absent in both bundle sheath chloroplasts and cells. The flash-induced 515 nm absorbance change of intact mesophyll chloroplasts showed a biphasic rise, previously known to be a characteristic only of intact algae. With bundle sheath chloroplasts or cells, no 515 nm signal could be detected. In the presence of 10 μmol l-1 phenazine methosulphate, bundle sheath chloroplasts exhibited a flash-induced 515 nm signal with a monophasic rise and amplitude comparable to that of the mesophyll chloroplasts. A similar signal was obtained with bundle sheath chloroplasts suspended in an extract prepared from the mesophyll tissue. Both the substrate stimulation of the CO2 fixation and the reconstitution of the 515 nm signal in bundle sheath chloroplasts by the mesophyll extract indicate the requirement of cooperation between the mesophyll and bundle sheath cells of maize leaves.

AB - A procedure was developed to isolate mesophyll and bundle sheath chloroplasts of a high degree of intactness and low cross-contamination. Light-induced 14CO2 fixation of isolated chloroplasts was similar to that of protoplasts and cells in that it was low and was stimulated by the addition of exogenous substrates. O2 evolution was absent in both bundle sheath chloroplasts and cells. The flash-induced 515 nm absorbance change of intact mesophyll chloroplasts showed a biphasic rise, previously known to be a characteristic only of intact algae. With bundle sheath chloroplasts or cells, no 515 nm signal could be detected. In the presence of 10 μmol l-1 phenazine methosulphate, bundle sheath chloroplasts exhibited a flash-induced 515 nm signal with a monophasic rise and amplitude comparable to that of the mesophyll chloroplasts. A similar signal was obtained with bundle sheath chloroplasts suspended in an extract prepared from the mesophyll tissue. Both the substrate stimulation of the CO2 fixation and the reconstitution of the 515 nm signal in bundle sheath chloroplasts by the mesophyll extract indicate the requirement of cooperation between the mesophyll and bundle sheath cells of maize leaves.

KW - Bundle sheath

KW - Chloroplasts

KW - Mesophyll

KW - Photosynthetics (C)

KW - Protoplasts

UR - http://www.scopus.com/inward/record.url?scp=0004340341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0004340341&partnerID=8YFLogxK

U2 - 10.1007/BF00388338

DO - 10.1007/BF00388338

M3 - Article

VL - 141

SP - 239

EP - 244

JO - Planta

JF - Planta

SN - 0032-0935

IS - 3

ER -