Functional assessment of the pathogenesis-related protein PR-1b in barley

Holger Schultheiss, Cornelia Dechert, Lorant Király, J. Fodor, Kathrin Michel, Karl Heinz Kogel, Ralph Hückelhoven

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The pathogenesis-related protein 1 (PR-1b) of barley (Hordeum vulgare L.) is a marker for the attack by the powdery mildew fungus (Blumeria graminis f.sp. hordei, Bgh) and other pathogens. PR-1b consists of 164 amino acids and has a potential signal peptide for export into the cell wall. Here, we show that PR-1b is differentially expressed in near-isogenic barley lines exhibiting various forms of defence phenotypes including papilla formation and the hypersensitive cell death. To elucidate PR-1b function, we transiently silenced PR-1 expression by double stranded RNA (dsRNA) interference in the moderately susceptible barley double mutant line A89 (genotype: mlo5-ror1), which shows a papillae-based defence phenotype. Upon bombardment of leaf segments with PR-1b dsRNA and a GFP marker gene construct, Bgh slightly more frequently penetrated the plant cell wall of transformed epidermal cells relative to cells bombarded with human control dsRNA. We conclude that PR-1b contributes to penetration resistance to the powdery mildew fungus in barley. We also observed that PR-1b expression correlates with the production of H2C2 in responses to Bgh and Bipolaris sorokiniana and was induced upon infiltration of the H2O2 producing mixture of glucose and glucose oxidase.

Original languageEnglish
Pages (from-to)1275-1280
Number of pages6
JournalPlant Science
Volume165
Issue number6
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Functional assessment
pathogenesis-related proteins
Double-Stranded RNA
Hordeum
double-stranded RNA
barley
Fungi
resistance to penetration
powdery mildew
Glucose Oxidase
Proteins
Cell Wall
cell walls
Pathogens
Cell death
Protein Sorting Signals
Blumeria graminis f. sp. hordei
Infiltration
phenotype
glucose oxidase

Keywords

  • Gene silencing
  • MLO
  • Pathogen
  • Reactive oxygen intermediates
  • Resistance
  • Suppression

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Schultheiss, H., Dechert, C., Király, L., Fodor, J., Michel, K., Kogel, K. H., & Hückelhoven, R. (2003). Functional assessment of the pathogenesis-related protein PR-1b in barley. Plant Science, 165(6), 1275-1280. https://doi.org/10.1016/S0168-9452(03)00336-4

Functional assessment of the pathogenesis-related protein PR-1b in barley. / Schultheiss, Holger; Dechert, Cornelia; Király, Lorant; Fodor, J.; Michel, Kathrin; Kogel, Karl Heinz; Hückelhoven, Ralph.

In: Plant Science, Vol. 165, No. 6, 12.2003, p. 1275-1280.

Research output: Contribution to journalArticle

Schultheiss, H, Dechert, C, Király, L, Fodor, J, Michel, K, Kogel, KH & Hückelhoven, R 2003, 'Functional assessment of the pathogenesis-related protein PR-1b in barley', Plant Science, vol. 165, no. 6, pp. 1275-1280. https://doi.org/10.1016/S0168-9452(03)00336-4
Schultheiss H, Dechert C, Király L, Fodor J, Michel K, Kogel KH et al. Functional assessment of the pathogenesis-related protein PR-1b in barley. Plant Science. 2003 Dec;165(6):1275-1280. https://doi.org/10.1016/S0168-9452(03)00336-4
Schultheiss, Holger ; Dechert, Cornelia ; Király, Lorant ; Fodor, J. ; Michel, Kathrin ; Kogel, Karl Heinz ; Hückelhoven, Ralph. / Functional assessment of the pathogenesis-related protein PR-1b in barley. In: Plant Science. 2003 ; Vol. 165, No. 6. pp. 1275-1280.
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