Functional and organisational change of photosystem II in poplar thylakoids under Cd stress (Dissipative PSII centres in Cd treated poplar thylakoids)

Ádám Solti, Judit Szucs, Brigitta Basa, Éva Sárvári

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cd contamination of soils and its easy uptake and translocation in plants cause serious disfunctions in plant metabolism, including photosynthesis. Thus, retarded development and damage of thylakoids come in to prominence, which may be accompanied by an acclimation of the thylakoid structure and function to the changed circumstances. To elucidate these alterations, we studied Cd induced organizational/compositional changes of thylakoid supercomplexes, and parallel changes in the excitation quenching. Poplar (Populus glauca var. 'Kopeczkii') plants were grown on 1/4 strength Hoagland solution (iron source: Fe (III)-citrate), and treated with or without 10 μM Cd(NO 3)2 from their four-leaf stage for two weeks. The first leaf that developed entirely under the treatment (sixth leaf) was used for isolation of thylakoid complexes and measurement of fluorescence induction in vivo. Mildly solubilised thylakoid macrocomplexes of control and Cd treated plants were analysed by gentle blue-native PAGE. The protein composition of the corresponding (super)complexes was similar in control and Cd treated samples. However, the ratio of photosystem (PS) II complexes being in higher aggregation state increased under Cd treatment. In parallel, a high relative amount of photoinhibited PS II centres and a significantly elevated dissipation of energy by inactive PS II centres were found in the treated samples. It was concluded that PS II aggregates could be involved in energy dissipation as heat under Cd stress.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalCereal Research Communications
Volume37
Issue numberSUPPL.1
DOIs
Publication statusPublished - Dec 31 2009

Keywords

  • Blue-native Page
  • Cadmium
  • Chlorophyll-protein complex
  • Photoinhibition
  • Photosynthesis

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics

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