Functional and morphological assessment of early impairment of airway function in a rat model of emphysema

J. Tolnai, M. V. Szabari, G. Albu, B. A. Maár, H. Parameswaran, E. Bartolák-Suki, B. Suki, Z. Hantos

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10 Citations (Scopus)

Abstract

Functional and morphological assessment of early impairment of airway function in a rat model of emphysema. J Appl Physiol 112: 1932-1939, 2012. First published March 22, 2012; doi:10.1152/japplphysiol.00587.2011.-The aim of this study was to evaluate airway structure-function relations in elastase-induced emphysema in rats. Sprague-Dawley rats were treated intratracheally with 50 IU porcine pancreatic elastase (PPE, n = 8) or saline (controls, n = 6). Six weeks later, lung volumes [functional residual capacity (FRC), residual volume (RV), and total lung capacity (TLC)] and low-frequency impedance parameters (Newtonian resistance, RN; tissue damping; tissue elastance, H) were measured, and tracheal sounds were recorded during slow inflation to TLC following in vivo degassing. The lungs were fixed and stained for standard morphometry, elastin, and collagen. In the PPE group, FRC and RV were higher [4.53 ± 0.7 (SD) vs. 3.28 ± 0.45 ml; P = 0.003 and 1.06 ± 0.35 vs. 0.69 ± 0.18 ml; P = 0.036, respectively], and H was smaller in the PPE-treated rats than in the controls (1,344 ± 216 vs. 2,178 ± 305 cmH2 O/l; P < 0.001), whereas there was no difference in RN. The average number of crackles per inflation was similar in the two groups; however, the crackle size distributions were different and the lower knee of the pressure-volume curves was higher in the PPE group. Microscopic images revealed different alveolar size distributions but similar bronchial diameters in the two groups. The treatment caused a slight but significant decrease in the numbers of alveolar attachments, no difference in elastin and slightly increased mean level and heterogeneity of collagen in the bronchial walls. These results suggest that tissue destruction did not affect the conventionally assessed airway resistance in this emphysema model, whereas the alterations in the recruitment dynamics can be an early manifestation of impaired airway function.

Original languageEnglish
Pages (from-to)1932-1939
Number of pages8
JournalJournal of Applied Physiology
Volume112
Issue number11
DOIs
Publication statusPublished - Jun 1 2012

Keywords

  • Crackles
  • Elastase
  • Lung volumes
  • Morphometry
  • Respiratory mechanics

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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    Tolnai, J., Szabari, M. V., Albu, G., Maár, B. A., Parameswaran, H., Bartolák-Suki, E., Suki, B., & Hantos, Z. (2012). Functional and morphological assessment of early impairment of airway function in a rat model of emphysema. Journal of Applied Physiology, 112(11), 1932-1939. https://doi.org/10.1152/japplphysiol.00587.2011