Functional analyses indicate a pathogenic role of factor H autoantibodies in atypical haemolytic uraemic syndrome

Stefanie Strobel, Peter F. Hoyer, Christoph J. MacHe, E. Sulyok, Wei Shih Liu, Heiko Richter, Martin Oppermann, Peter F. Zipfel, Mihály Józsi

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Background. Atypical haemolytic uraemic syndrome (aHUS) is associated with defective complement regulation. Recently, an autoimmune aHUS form has been described that is associated with complement factor H (CFH) autoantibodies. The aim of this study was to address the pathologic relevance of CFH autoantibodies in aHUS.Methods. CFH autoantibodies were identified and antibody levels were analysed in three aHUS patients during the disease course by the ELISA method. Epitope mapping was performed using recombinant factor H fragments and domain-mapped monoclonal antibodies. The effect of the antibodies on cell-protective activity of CFH was measured by haemolytic assays. CFH:autoantibody complexes were analysed by ELISA.Results. All three autoantibodies bound to the C-terminal domain of CFH, which is essential for CFH binding to cell surfaces. In patient 1, plasma exchanges and immune adsorption temporarily reduced the autoantibody titre and led to temporary clinical improvement. In patient 2, plasma exchanges and long-term immunosuppression strongly reduced the CFH autoantibody level, and induced a stable remission of aHUS. Patient 3 had lower autoantibody levels that decreased during the follow-up and is in good clinical condition. The patients' plasma samples caused enhanced lysis of sheep erythrocytes, and the degree of lysis correlated with the CFH autoantibody titre and the amount of CFH:autoantibody complexes. An addition of purified CFH to aHUS plasma or removal of IgG inhibited the haemolytic activity.Conclusion. These results support a direct role of the autoantibodies in aHUS pathology by inhibiting the regulatory function of CFH at cell surfaces and suggest that reduction of the autoantibody titre is beneficial for the patients.

Original languageEnglish
Pages (from-to)136-144
Number of pages9
JournalNephrology Dialysis Transplantation
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2010

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Complement Factor H
Autoantibodies
Plasma Exchange
Atypical Hemolytic Uremic Syndrome
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Antibodies
Immunosuppression
Adsorption

Keywords

  • Autoantibody
  • Complement
  • Factor H
  • Haemolytic uraemic syndrome

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Functional analyses indicate a pathogenic role of factor H autoantibodies in atypical haemolytic uraemic syndrome. / Strobel, Stefanie; Hoyer, Peter F.; MacHe, Christoph J.; Sulyok, E.; Liu, Wei Shih; Richter, Heiko; Oppermann, Martin; Zipfel, Peter F.; Józsi, Mihály.

In: Nephrology Dialysis Transplantation, Vol. 25, No. 1, 01.2010, p. 136-144.

Research output: Contribution to journalArticle

Strobel, S, Hoyer, PF, MacHe, CJ, Sulyok, E, Liu, WS, Richter, H, Oppermann, M, Zipfel, PF & Józsi, M 2010, 'Functional analyses indicate a pathogenic role of factor H autoantibodies in atypical haemolytic uraemic syndrome', Nephrology Dialysis Transplantation, vol. 25, no. 1, pp. 136-144. https://doi.org/10.1093/ndt/gfp388
Strobel, Stefanie ; Hoyer, Peter F. ; MacHe, Christoph J. ; Sulyok, E. ; Liu, Wei Shih ; Richter, Heiko ; Oppermann, Martin ; Zipfel, Peter F. ; Józsi, Mihály. / Functional analyses indicate a pathogenic role of factor H autoantibodies in atypical haemolytic uraemic syndrome. In: Nephrology Dialysis Transplantation. 2010 ; Vol. 25, No. 1. pp. 136-144.
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abstract = "Background. Atypical haemolytic uraemic syndrome (aHUS) is associated with defective complement regulation. Recently, an autoimmune aHUS form has been described that is associated with complement factor H (CFH) autoantibodies. The aim of this study was to address the pathologic relevance of CFH autoantibodies in aHUS.Methods. CFH autoantibodies were identified and antibody levels were analysed in three aHUS patients during the disease course by the ELISA method. Epitope mapping was performed using recombinant factor H fragments and domain-mapped monoclonal antibodies. The effect of the antibodies on cell-protective activity of CFH was measured by haemolytic assays. CFH:autoantibody complexes were analysed by ELISA.Results. All three autoantibodies bound to the C-terminal domain of CFH, which is essential for CFH binding to cell surfaces. In patient 1, plasma exchanges and immune adsorption temporarily reduced the autoantibody titre and led to temporary clinical improvement. In patient 2, plasma exchanges and long-term immunosuppression strongly reduced the CFH autoantibody level, and induced a stable remission of aHUS. Patient 3 had lower autoantibody levels that decreased during the follow-up and is in good clinical condition. The patients' plasma samples caused enhanced lysis of sheep erythrocytes, and the degree of lysis correlated with the CFH autoantibody titre and the amount of CFH:autoantibody complexes. An addition of purified CFH to aHUS plasma or removal of IgG inhibited the haemolytic activity.Conclusion. These results support a direct role of the autoantibodies in aHUS pathology by inhibiting the regulatory function of CFH at cell surfaces and suggest that reduction of the autoantibody titre is beneficial for the patients.",
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AU - Liu, Wei Shih

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AB - Background. Atypical haemolytic uraemic syndrome (aHUS) is associated with defective complement regulation. Recently, an autoimmune aHUS form has been described that is associated with complement factor H (CFH) autoantibodies. The aim of this study was to address the pathologic relevance of CFH autoantibodies in aHUS.Methods. CFH autoantibodies were identified and antibody levels were analysed in three aHUS patients during the disease course by the ELISA method. Epitope mapping was performed using recombinant factor H fragments and domain-mapped monoclonal antibodies. The effect of the antibodies on cell-protective activity of CFH was measured by haemolytic assays. CFH:autoantibody complexes were analysed by ELISA.Results. All three autoantibodies bound to the C-terminal domain of CFH, which is essential for CFH binding to cell surfaces. In patient 1, plasma exchanges and immune adsorption temporarily reduced the autoantibody titre and led to temporary clinical improvement. In patient 2, plasma exchanges and long-term immunosuppression strongly reduced the CFH autoantibody level, and induced a stable remission of aHUS. Patient 3 had lower autoantibody levels that decreased during the follow-up and is in good clinical condition. The patients' plasma samples caused enhanced lysis of sheep erythrocytes, and the degree of lysis correlated with the CFH autoantibody titre and the amount of CFH:autoantibody complexes. An addition of purified CFH to aHUS plasma or removal of IgG inhibited the haemolytic activity.Conclusion. These results support a direct role of the autoantibodies in aHUS pathology by inhibiting the regulatory function of CFH at cell surfaces and suggest that reduction of the autoantibody titre is beneficial for the patients.

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