Fumonisin B1 exposure increases Hsp70 expression in the lung and kidney of rats without inducing significant oxidative stress

Dániel J. Kócsó, Judit Szabó-Fodor, M. Mézes, K. Balogh, Szilamér Ferenczi, András Szabó, Brigitta Bóta, Melinda Kovács

Research output: Contribution to journalArticle

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Abstract

The objective of this experiment was to determine whether fumonisin B1 (FB1) added to the diet of rats in a dose of 50 mg/kg changes the production of heat shock protein 70 (Hsp70) in the lungs and kidney of rats. We also studied the effect of this mycotoxin on the antioxidant system of the body. Mature (8 weeks old) male Wistar Crl:WI BR rats (n = 6/group) were fed the toxin-containing diet for 5 days. FB1 resulted in a 7% body weight reduction without significantly changing the feed intake. Western blot analysis of the lungs and kidney demonstrated a substantial (1.4-fold and 1.8-fold, respectively) increase in Hsp70 expression. Alterations could not be detected in the clinical chemical parameters (total protein, albumin, total cholesterol, glucose, creatinine and urea concentrations, and aspartate aminotransferase activity). There was no statistically significant change in malondialdehyde concentrations and the measured antioxidant parameters (the amount of reduced glutathione, GSH and glutathione peroxidase activity, GPx) in the blood plasma, lung and kidney tissue. Thus, it can be concluded that FB1 did not induce oxidative stress in the lungs and kidney, but increased Hsp70 production.

Original languageEnglish
Pages (from-to)394-407
Number of pages14
JournalActa Veterinaria Hungarica
Volume66
Issue number3
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

fumonisin B1
HSP70 Heat-Shock Proteins
Oxidative Stress
oxidative stress
lungs
kidneys
Kidney
Lung
rats
glutathione peroxidase
Antioxidants
Diet
antioxidants
Mycotoxins
Glutathione Peroxidase
Aspartate Aminotransferases
Malondialdehyde
blood plasma
aspartate transaminase
diet

Keywords

  • Antioxidants
  • Clinical chemical parameters
  • Fumonisin Brat
  • Hsp70
  • Oxidative stress

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Fumonisin B1 exposure increases Hsp70 expression in the lung and kidney of rats without inducing significant oxidative stress. / Kócsó, Dániel J.; Szabó-Fodor, Judit; Mézes, M.; Balogh, K.; Ferenczi, Szilamér; Szabó, András; Bóta, Brigitta; Kovács, Melinda.

In: Acta Veterinaria Hungarica, Vol. 66, No. 3, 01.09.2018, p. 394-407.

Research output: Contribution to journalArticle

Kócsó, Dániel J. ; Szabó-Fodor, Judit ; Mézes, M. ; Balogh, K. ; Ferenczi, Szilamér ; Szabó, András ; Bóta, Brigitta ; Kovács, Melinda. / Fumonisin B1 exposure increases Hsp70 expression in the lung and kidney of rats without inducing significant oxidative stress. In: Acta Veterinaria Hungarica. 2018 ; Vol. 66, No. 3. pp. 394-407.
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