Fully on-line hyphenation of an experimental OPLC separation unit with diode-array detection and mass-spectrometry (OPLC-DAD-MS) for analysis of xanthine compounds

E. Mincsovics, Katalin Pápai, K. Ludányi, Ádám Zoltán Dávid, M. Budai, I. Antal, I. Klebovich

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The newly developed experimental OPLC separation unit 100 (OSU 100) has been used for fully on-line multiple hyphenation using the one-channel flowing eluent wall (FEW) arrangement at the inlet. OSU 100-UV and OSU 100-DAD-ESI-MS with manual injector or autosampler were used to test the connected systems (OPLC-UV; OPLC-DAD-ESI-MS), using xanthine standards (caffeine, theophylline, theobromine) and green tea leaf extract as model compounds. The mobile phase chloroform-trifluoroacetic acid-acetonitrile-methanol, 76:4:6.67:13.33 (ν/ν) is suitable for rapid and selective separation, DAD, and ESI-MS of xanthines. The detection sensitivity of OPLC-UV or OPLC-DAD was increased by hyphenation with ESI-MS coupled in series. In contrast with DAD, the extracted ion chromatogram (m/z = 181.1 Da) after background subtraction yields readily measurable peaks from the small amounts of theophylline and theobromine in tea leaf extract. Analysis of xanthines was achieved within 10 min with less than 2 mL mobile phase. Owing to the openable adsorbent layer in OPLC the bed conditions can, moreover, be checked off-line after single or multiple fully online separations.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume21
Issue number5
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Xanthines
Theobromine
Xanthine
Tea
Theophylline
Mass spectrometry
Mass Spectrometry
Diodes
Trifluoroacetic Acid
Chloroform
Caffeine
Methanol
Ions
Adsorbents

Keywords

  • Fully on-line multiple hyphenation
  • Green tea
  • OPLC separation unit 100 (OSU 100)
  • OPLC-DAD-ESI-MS
  • Overpressured-layer chromatography (OPLC)
  • Xanthines (caffeine, theophylline, theobromine)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

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title = "Fully on-line hyphenation of an experimental OPLC separation unit with diode-array detection and mass-spectrometry (OPLC-DAD-MS) for analysis of xanthine compounds",
abstract = "The newly developed experimental OPLC separation unit 100 (OSU 100) has been used for fully on-line multiple hyphenation using the one-channel flowing eluent wall (FEW) arrangement at the inlet. OSU 100-UV and OSU 100-DAD-ESI-MS with manual injector or autosampler were used to test the connected systems (OPLC-UV; OPLC-DAD-ESI-MS), using xanthine standards (caffeine, theophylline, theobromine) and green tea leaf extract as model compounds. The mobile phase chloroform-trifluoroacetic acid-acetonitrile-methanol, 76:4:6.67:13.33 (ν/ν) is suitable for rapid and selective separation, DAD, and ESI-MS of xanthines. The detection sensitivity of OPLC-UV or OPLC-DAD was increased by hyphenation with ESI-MS coupled in series. In contrast with DAD, the extracted ion chromatogram (m/z = 181.1 Da) after background subtraction yields readily measurable peaks from the small amounts of theophylline and theobromine in tea leaf extract. Analysis of xanthines was achieved within 10 min with less than 2 mL mobile phase. Owing to the openable adsorbent layer in OPLC the bed conditions can, moreover, be checked off-line after single or multiple fully online separations.",
keywords = "Fully on-line multiple hyphenation, Green tea, OPLC separation unit 100 (OSU 100), OPLC-DAD-ESI-MS, Overpressured-layer chromatography (OPLC), Xanthines (caffeine, theophylline, theobromine)",
author = "E. Mincsovics and Katalin P{\'a}pai and K. Lud{\'a}nyi and D{\'a}vid, {{\'A}d{\'a}m Zolt{\'a}n} and M. Budai and I. Antal and I. Klebovich",
year = "2008",
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journal = "Journal of Planar Chromatography - Modern TLC",
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T1 - Fully on-line hyphenation of an experimental OPLC separation unit with diode-array detection and mass-spectrometry (OPLC-DAD-MS) for analysis of xanthine compounds

AU - Mincsovics, E.

AU - Pápai, Katalin

AU - Ludányi, K.

AU - Dávid, Ádám Zoltán

AU - Budai, M.

AU - Antal, I.

AU - Klebovich, I.

PY - 2008/10

Y1 - 2008/10

N2 - The newly developed experimental OPLC separation unit 100 (OSU 100) has been used for fully on-line multiple hyphenation using the one-channel flowing eluent wall (FEW) arrangement at the inlet. OSU 100-UV and OSU 100-DAD-ESI-MS with manual injector or autosampler were used to test the connected systems (OPLC-UV; OPLC-DAD-ESI-MS), using xanthine standards (caffeine, theophylline, theobromine) and green tea leaf extract as model compounds. The mobile phase chloroform-trifluoroacetic acid-acetonitrile-methanol, 76:4:6.67:13.33 (ν/ν) is suitable for rapid and selective separation, DAD, and ESI-MS of xanthines. The detection sensitivity of OPLC-UV or OPLC-DAD was increased by hyphenation with ESI-MS coupled in series. In contrast with DAD, the extracted ion chromatogram (m/z = 181.1 Da) after background subtraction yields readily measurable peaks from the small amounts of theophylline and theobromine in tea leaf extract. Analysis of xanthines was achieved within 10 min with less than 2 mL mobile phase. Owing to the openable adsorbent layer in OPLC the bed conditions can, moreover, be checked off-line after single or multiple fully online separations.

AB - The newly developed experimental OPLC separation unit 100 (OSU 100) has been used for fully on-line multiple hyphenation using the one-channel flowing eluent wall (FEW) arrangement at the inlet. OSU 100-UV and OSU 100-DAD-ESI-MS with manual injector or autosampler were used to test the connected systems (OPLC-UV; OPLC-DAD-ESI-MS), using xanthine standards (caffeine, theophylline, theobromine) and green tea leaf extract as model compounds. The mobile phase chloroform-trifluoroacetic acid-acetonitrile-methanol, 76:4:6.67:13.33 (ν/ν) is suitable for rapid and selective separation, DAD, and ESI-MS of xanthines. The detection sensitivity of OPLC-UV or OPLC-DAD was increased by hyphenation with ESI-MS coupled in series. In contrast with DAD, the extracted ion chromatogram (m/z = 181.1 Da) after background subtraction yields readily measurable peaks from the small amounts of theophylline and theobromine in tea leaf extract. Analysis of xanthines was achieved within 10 min with less than 2 mL mobile phase. Owing to the openable adsorbent layer in OPLC the bed conditions can, moreover, be checked off-line after single or multiple fully online separations.

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KW - OPLC-DAD-ESI-MS

KW - Overpressured-layer chromatography (OPLC)

KW - Xanthines (caffeine, theophylline, theobromine)

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U2 - 10.1556/JPC.21.2008.5.8

DO - 10.1556/JPC.21.2008.5.8

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JO - Journal of Planar Chromatography - Modern TLC

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SN - 0933-4173

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