Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

A. Bálint, Gyula Balka, Péter Horváth, S. Kecskeméti, A. Dán, Attila Farsang, L. Szeredi, K. Bányai, Dániel Bartha, F. Olasz, Sándor Belák, Z. Zádori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalArchives of Virology
Volume160
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Porcine respiratory and reproductive syndrome virus
Sequence Analysis
Swine
Genome
Amino Acid Substitution
Genetic Recombination
Disease Outbreaks
Epitopes
Fever
Vaccines
Viruses
Pressure
Mutation

Keywords

  • Complete genome
  • Epitope
  • PRRSV
  • Sequencing

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease. / Bálint, A.; Balka, Gyula; Horváth, Péter; Kecskeméti, S.; Dán, A.; Farsang, Attila; Szeredi, L.; Bányai, K.; Bartha, Dániel; Olasz, F.; Belák, Sándor; Zádori, Z.

In: Archives of Virology, Vol. 160, No. 2, 2014, p. 417-422.

Research output: Contribution to journalArticle

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AU - Kecskeméti, S.

AU - Dán, A.

AU - Farsang, Attila

AU - Szeredi, L.

AU - Bányai, K.

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