Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla Anguilla showing signs of cauliflower disease

Andor Doszpoly, Zoltán L. Tarján, Róbert Glávits, Tamás Müller, Mária Benko

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An adult European eel Anguilla anguilla, showing typical signs of the so-called cauli - flower disease, was subjected to pathological and molecular virological examinations. Samples taken from internal organs and the polypoid proliferative tissue from the mouth were examined by PCR for the detection of several viruses. Positive results were obtained with a nested PCR targeting the rep gene of circoviruses. Analysis of the partial rep sequence indicated the presence of a putative novel circovirus, but attempts to isolate it remained unsuccessful. The missing part of the genome was acquired by an inverse nested PCR with 2 specific primer pairs, designed from the newly determined rep sequence, followed by genome walking. The circular full genome was found to consist of 1378 nt (GenBank accession no. KC469701). Two oppositely oriented open reading frames (ORFs) were present, of which one was unambiguously identified as a circoviral rep gene. However, the predicted product of the other ORF, though it is a clear positional counterpart of the cap genes, showed no obvious homology to any known circoviral capsid proteins. A stem-loop-like element in the intergenic region between the 5' ends of the ORFs was also found. Phylogenetic calculations indicated that the novel virus belongs to the genus Circovirus in the family Circoviridae. The relative amount of the viral DNA in the organ samples was estimated by quantitative real-time PCR. The results suggested that the examined fish was caught in an active viremic state, although the role of this circovirus in the etiology of the cauliflower diseases could not be ascertained.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalDiseases of Aquatic Organisms
Volume109
Issue number2
DOIs
Publication statusPublished - May 13 2014

Keywords

  • Cauliflower disease
  • Complete genome
  • Fish circovirus
  • Phylogeny
  • Stomatopapilloma

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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