Full genome characterization of human Rotavirus A strains isolated in Cameroon, 2010-2011: Diverse combinations of the G and P genes and lack of reassortment of the backbone genes

Valentine Ngum Ndze, Mathew Dioh Esona, Eric Akum Achidi, Kamga Hortense Gonsu, Renáta Dóró, Szilvia Marton, Szilvia Farkas, Marxcel Bong Ngeng, Akum Felix Ngu, Marie Therese Obama-Abena, K. Bányai

Research output: Contribution to journalArticle

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Abstract

Over the past few years whole genome sequencing of rotaviruses has become a routine laboratory method in many strain surveillance studies. To study the molecular evolutionary pattern of representative Cameroonian Rotavirus A (RVA) strains, the semiconductor sequencing approach was used following random amplification of genomic RNA. In total, 31 RVA strains collected during 2010-2011 in three Cameroonian study sites located 120 to 1240. km from each other were sequenced and analyzed. Sequence analysis of the randomly selected representative strains showed that 18 RVAs were Wa-like, expressing G1P[6], G12P[6], or G12P[8] neutralization antigens on the genotype 1 genomic constellation (I1-R1-C1-M1-A1-N1-T1-E1-H1), whereas 13 other strains were DS-1-like, expressing G2P[4], G2P[6], G3P[6], and G6P[6] on the genotype 2 genomic constellation (I2-R2-C2-M2-A2-N2-T2-E2-H2). No inter-genogroup reassortment in the backbone genes was observed. Phylogenetic analysis of the Cameroonian G6P[6] strains indicated the separation of the strains identified in the Far North region (Maroua) and the Northwest region (Bamenda and Esu) into two branches that is consistent with multiple introductions of G6P[6] strains into this country. The present whole genome based molecular characterization study indicates that the emerging G6P[6] strain is fully heterotypic to Rotarix, the vaccine introduced during 2014 in childhood immunization program in Cameroon. Continuous strain monitoring is therefore needed in this area and elsewhere to see if G6s, besides genotype G1 to G4, G8, G9 and G12, may become a new, regionally important genotype in the post vaccine licensure era in Africa.

Original languageEnglish
Pages (from-to)537-560
Number of pages24
JournalInfection, Genetics and Evolution
Volume28
DOIs
Publication statusPublished - Dec 1 2014

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Rotavirus A
Cameroon
Rotavirus
Human Genome
genotype
genome
Genotype
genomics
gene
vaccine
Genes
genes
Vaccines
laboratory method
immunization
Genome
neutralization
antigen
Immunization Programs
Semiconductors

Keywords

  • Cameroon
  • Ion Torrent PGM
  • Phylogeny
  • Rotavirus A genomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Full genome characterization of human Rotavirus A strains isolated in Cameroon, 2010-2011 : Diverse combinations of the G and P genes and lack of reassortment of the backbone genes. / Ndze, Valentine Ngum; Esona, Mathew Dioh; Achidi, Eric Akum; Gonsu, Kamga Hortense; Dóró, Renáta; Marton, Szilvia; Farkas, Szilvia; Ngeng, Marxcel Bong; Ngu, Akum Felix; Obama-Abena, Marie Therese; Bányai, K.

In: Infection, Genetics and Evolution, Vol. 28, 01.12.2014, p. 537-560.

Research output: Contribution to journalArticle

Ndze, Valentine Ngum ; Esona, Mathew Dioh ; Achidi, Eric Akum ; Gonsu, Kamga Hortense ; Dóró, Renáta ; Marton, Szilvia ; Farkas, Szilvia ; Ngeng, Marxcel Bong ; Ngu, Akum Felix ; Obama-Abena, Marie Therese ; Bányai, K. / Full genome characterization of human Rotavirus A strains isolated in Cameroon, 2010-2011 : Diverse combinations of the G and P genes and lack of reassortment of the backbone genes. In: Infection, Genetics and Evolution. 2014 ; Vol. 28. pp. 537-560.
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AU - Achidi, Eric Akum

AU - Gonsu, Kamga Hortense

AU - Dóró, Renáta

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