FTY720 (Gilenya) treatment prevents spontaneous autoimmune myocarditis and dilated cardiomyopathy in transgenic HLA-DQ8-BALB/c mice

F. Boldizsár, Oktavia Tarjanyi, Katalin Olasz, Akos Hegyi, Katalin Mikecz, Tibor T. Glant, Tibor A. Rauch

Research output: Contribution to journalArticle

Abstract

Although dilated cardiomyopathy (DCM) is often caused by viral infections, it frequently involves autoimmune mechanisms associated with particular HLA-DR and DQ alleles. Our homozygous HLA-DQ8Ab0 transgenic mice in the BALB/c background (HLA-DQ8BALB/c-Tg) developed early and progressive fatal heart failure from 4 to 5 weeks of age. Clinical signs of the disease included cyanotic eyes, tachycardia with dyspnea (from pale to cyanotic limbs), and terminal whole body edema. Sick mice had extremely dilated hearts, enlarged liver and spleen, and pleural/peritoneal effusion. Histology of the heart showed extensive heart muscle destruction with signs of fibrosis. The autoimmune nature of the disease was shown by high titers of antimyosin antibodies in the sera and IgG deposits in sick heart muscles, as well as focal neutrophil, T cell, and macrophage infiltration of the heart muscle. The sera of the sick mice showed a granular staining pattern on sections of healthy heart muscle. Quantitative analyses of DCM-specific gene expression studies revealed that sets of genes are involved in inflammation, hypoxia, and fibrosis. Treatment with FTY720 (Fingolimod/Gilenya) protected animals from the development of cardiomyopathy. HLA-DQ8BALB/c-Tg mice represent a spontaneous autoimmune myocarditis model that may provide a useful tool for studying the autoimmune mechanism of DCM and testing immunosuppressive drugs.

Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalCardiovascular Pathology
Volume25
Issue number5
DOIs
Publication statusPublished - Sep 1 2016

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Myocarditis
Dilated Cardiomyopathy
Myocardium
Fibrosis
HLA-DQ Antigens
Hepatomegaly
Ascitic Fluid
Splenomegaly
HLA-DR Antigens
Virus Diseases
Pleural Effusion
Therapeutics
Immunosuppressive Agents
Serum
Cardiomyopathies
Tachycardia
Dyspnea
Transgenic Mice
Autoimmune Diseases
Edema

Keywords

  • Autoimmune cardiomyopathy
  • FTY720
  • Mouse model

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Cite this

FTY720 (Gilenya) treatment prevents spontaneous autoimmune myocarditis and dilated cardiomyopathy in transgenic HLA-DQ8-BALB/c mice. / Boldizsár, F.; Tarjanyi, Oktavia; Olasz, Katalin; Hegyi, Akos; Mikecz, Katalin; Glant, Tibor T.; Rauch, Tibor A.

In: Cardiovascular Pathology, Vol. 25, No. 5, 01.09.2016, p. 353-361.

Research output: Contribution to journalArticle

Boldizsár, F. ; Tarjanyi, Oktavia ; Olasz, Katalin ; Hegyi, Akos ; Mikecz, Katalin ; Glant, Tibor T. ; Rauch, Tibor A. / FTY720 (Gilenya) treatment prevents spontaneous autoimmune myocarditis and dilated cardiomyopathy in transgenic HLA-DQ8-BALB/c mice. In: Cardiovascular Pathology. 2016 ; Vol. 25, No. 5. pp. 353-361.
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