FTIR detection of an enzyme-bound organometallic carbonyl probe in the presence of the unbound probe molecule

Christopher E. Anson, Colin S. Creaser, Orsolya Egyed, Mark A. Fey, G. Richard Stephenson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

High-frequency shoulders on νs(CO) and νas(CO) bands in the FTIR spectra of an organometallic probe derived from tricarbonyl(1-4-η-5-pyridiniocyclohexa-1,3-diene)iron hexafluorophosphate have been observed on incubation with α-chymotrypsin and were assigned to an iron complex covalently bonded to enzyme-NH2 groups; curve-fitting analysis of the spectra enabled the bound complex to be detected even in the presence of an excess of the unbound probe and its FTIR spectrum to be calculated and compared with spectral data arising from similar interactions between the tricarbonyliron moiety and 12 amino acids and polylysine.

Original languageEnglish
Pages (from-to)39-40
Number of pages2
JournalJournal of the Chemical Society, Chemical Communications
Issue number1
DOIs
Publication statusPublished - Dec 1 1994

ASJC Scopus subject areas

  • Molecular Medicine

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