From dendrites to networks

Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology

Jesse H. Goldberg, Farid Hamzei-Sichani, Jason MacLean, G. Tamás, Rochelle Urban, Rafael Yuste

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Recently, advances in optical imaging of the living brain slice preparation have permitted neuronal circuitry to be examined at multiple levels, ranging from individual synaptic contacts on dendrites to whole populations of neurons in a network. In this chapter, we describe three techniques that, together, enable a powerful dissection of neuronal circuits across multiple space scales. We describe methods for (1) combining whole-cell recording with two-photon calcium imaging and electron microscopic reconstruction to examine the functions of individual synapses and dendrites during synaptic stimulation, (2) imaging hundreds of neurons in the brain slice simultaneously to examine the spatiotemporal dynamics of activity in living neuronal networks, and (3) performing an unbiased, quantitative analysis of neuronal morphology that is increasingly necessary in light of the multiparametric structural diversity of distinct neuronal subclasses.

Original languageEnglish
Title of host publicationNeuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems
PublisherSpringer US
Pages452-476
Number of pages25
ISBN (Print)9780387289427, 0387289410, 9780387289410
DOIs
Publication statusPublished - 2006

Fingerprint

Dendrites
Principal Component Analysis
Neurons
Optical Imaging
Brain
Patch-Clamp Techniques
Photons
Synapses
Dissection
Electrons
Calcium
Population

Keywords

  • Cluster analysis
  • Dendrite
  • Imaging
  • Microdomain
  • Network
  • Principal component analysis
  • Two-photon calcium

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)

Cite this

Goldberg, J. H., Hamzei-Sichani, F., MacLean, J., Tamás, G., Urban, R., & Yuste, R. (2006). From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology. In Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems (pp. 452-476). Springer US. https://doi.org/10.1007/0-387-28942-9_15

From dendrites to networks : Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology. / Goldberg, Jesse H.; Hamzei-Sichani, Farid; MacLean, Jason; Tamás, G.; Urban, Rochelle; Yuste, Rafael.

Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems. Springer US, 2006. p. 452-476.

Research output: Chapter in Book/Report/Conference proceedingChapter

Goldberg, JH, Hamzei-Sichani, F, MacLean, J, Tamás, G, Urban, R & Yuste, R 2006, From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology. in Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems. Springer US, pp. 452-476. https://doi.org/10.1007/0-387-28942-9_15
Goldberg JH, Hamzei-Sichani F, MacLean J, Tamás G, Urban R, Yuste R. From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology. In Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems. Springer US. 2006. p. 452-476 https://doi.org/10.1007/0-387-28942-9_15
Goldberg, Jesse H. ; Hamzei-Sichani, Farid ; MacLean, Jason ; Tamás, G. ; Urban, Rochelle ; Yuste, Rafael. / From dendrites to networks : Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology. Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems. Springer US, 2006. pp. 452-476
@inbook{354f108dd57649bc9ec7f6f3444611ab,
title = "From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology",
abstract = "Recently, advances in optical imaging of the living brain slice preparation have permitted neuronal circuitry to be examined at multiple levels, ranging from individual synaptic contacts on dendrites to whole populations of neurons in a network. In this chapter, we describe three techniques that, together, enable a powerful dissection of neuronal circuits across multiple space scales. We describe methods for (1) combining whole-cell recording with two-photon calcium imaging and electron microscopic reconstruction to examine the functions of individual synapses and dendrites during synaptic stimulation, (2) imaging hundreds of neurons in the brain slice simultaneously to examine the spatiotemporal dynamics of activity in living neuronal networks, and (3) performing an unbiased, quantitative analysis of neuronal morphology that is increasingly necessary in light of the multiparametric structural diversity of distinct neuronal subclasses.",
keywords = "Cluster analysis, Dendrite, Imaging, Microdomain, Network, Principal component analysis, Two-photon calcium",
author = "Goldberg, {Jesse H.} and Farid Hamzei-Sichani and Jason MacLean and G. Tam{\'a}s and Rochelle Urban and Rafael Yuste",
year = "2006",
doi = "10.1007/0-387-28942-9_15",
language = "English",
isbn = "9780387289427",
pages = "452--476",
booktitle = "Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems",
publisher = "Springer US",

}

TY - CHAP

T1 - From dendrites to networks

T2 - Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology

AU - Goldberg, Jesse H.

AU - Hamzei-Sichani, Farid

AU - MacLean, Jason

AU - Tamás, G.

AU - Urban, Rochelle

AU - Yuste, Rafael

PY - 2006

Y1 - 2006

N2 - Recently, advances in optical imaging of the living brain slice preparation have permitted neuronal circuitry to be examined at multiple levels, ranging from individual synaptic contacts on dendrites to whole populations of neurons in a network. In this chapter, we describe three techniques that, together, enable a powerful dissection of neuronal circuits across multiple space scales. We describe methods for (1) combining whole-cell recording with two-photon calcium imaging and electron microscopic reconstruction to examine the functions of individual synapses and dendrites during synaptic stimulation, (2) imaging hundreds of neurons in the brain slice simultaneously to examine the spatiotemporal dynamics of activity in living neuronal networks, and (3) performing an unbiased, quantitative analysis of neuronal morphology that is increasingly necessary in light of the multiparametric structural diversity of distinct neuronal subclasses.

AB - Recently, advances in optical imaging of the living brain slice preparation have permitted neuronal circuitry to be examined at multiple levels, ranging from individual synaptic contacts on dendrites to whole populations of neurons in a network. In this chapter, we describe three techniques that, together, enable a powerful dissection of neuronal circuits across multiple space scales. We describe methods for (1) combining whole-cell recording with two-photon calcium imaging and electron microscopic reconstruction to examine the functions of individual synapses and dendrites during synaptic stimulation, (2) imaging hundreds of neurons in the brain slice simultaneously to examine the spatiotemporal dynamics of activity in living neuronal networks, and (3) performing an unbiased, quantitative analysis of neuronal morphology that is increasingly necessary in light of the multiparametric structural diversity of distinct neuronal subclasses.

KW - Cluster analysis

KW - Dendrite

KW - Imaging

KW - Microdomain

KW - Network

KW - Principal component analysis

KW - Two-photon calcium

UR - http://www.scopus.com/inward/record.url?scp=79958174705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958174705&partnerID=8YFLogxK

U2 - 10.1007/0-387-28942-9_15

DO - 10.1007/0-387-28942-9_15

M3 - Chapter

SN - 9780387289427

SN - 0387289410

SN - 9780387289410

SP - 452

EP - 476

BT - Neuroanatomical Tract-Tracing 3: Molecules, Neurons, and Systems

PB - Springer US

ER -