Fritless chromatographic microfluidic-based columns for chemical separations

A. Gáspár, Shima Baghdachi, Mark Goldberg, Schetema Stevens, Jaime Torres, Marisol Salgado, Frank A. Gomez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A study was conducted to demonstrate the development of fritless liquid chromatographic (LC) microfluidic-based columns for chemical separations. The study demonstrated the microfluidic-based LC technique to separate dyes using C18 particles as the stationary phase. The reversed-phase chromatographic packing material consisted of porous C18-modified 10-μm particles, while suspension of C18 beads was prepared in methanol by sonication. Poly(dimethylsiloxane) (PDMS) chips were also prepared by using a mold created by soft photolithography. The protein purification microchip consisted of a pattern of 100-μm-wide channels. The chip was rinsed with degassed methanol for three minutes to clean the wall and to remove any residual water from the channel. It was demonstrated that it was easy to pack C18 particles into the microfluidic channel due to its hydrophobicity and high elasticity.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalAmerican Laboratory
Volume40
Issue number18
Publication statusPublished - Oct 2008

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Microfluidics
Methanol
Protein Array Analysis
Aquaporins
Sonication
Elasticity
Liquids
Photolithography
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Purification
Suspensions
Fungi
Coloring Agents
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Gáspár, A., Baghdachi, S., Goldberg, M., Stevens, S., Torres, J., Salgado, M., & Gomez, F. A. (2008). Fritless chromatographic microfluidic-based columns for chemical separations. American Laboratory, 40(18), 13-16.

Fritless chromatographic microfluidic-based columns for chemical separations. / Gáspár, A.; Baghdachi, Shima; Goldberg, Mark; Stevens, Schetema; Torres, Jaime; Salgado, Marisol; Gomez, Frank A.

In: American Laboratory, Vol. 40, No. 18, 10.2008, p. 13-16.

Research output: Contribution to journalArticle

Gáspár, A, Baghdachi, S, Goldberg, M, Stevens, S, Torres, J, Salgado, M & Gomez, FA 2008, 'Fritless chromatographic microfluidic-based columns for chemical separations', American Laboratory, vol. 40, no. 18, pp. 13-16.
Gáspár A, Baghdachi S, Goldberg M, Stevens S, Torres J, Salgado M et al. Fritless chromatographic microfluidic-based columns for chemical separations. American Laboratory. 2008 Oct;40(18):13-16.
Gáspár, A. ; Baghdachi, Shima ; Goldberg, Mark ; Stevens, Schetema ; Torres, Jaime ; Salgado, Marisol ; Gomez, Frank A. / Fritless chromatographic microfluidic-based columns for chemical separations. In: American Laboratory. 2008 ; Vol. 40, No. 18. pp. 13-16.
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