Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues

Hazem M. Kalaji, G. Schansker, Richard J. Ladle, Vasilij Goltsev, Karolina Bosa, Suleyman I. Allakhverdiev, Marian Brestic, Filippo Bussotti, Angeles Calatayud, Piotr Dąbrowski, Nabil I. Elsheery, Lorenzo Ferroni, Lucia Guidi, Sander W. Hogewoning, Anjana Jajoo, Amarendra N. Misra, Sergio G. Nebauer, Simonetta Pancaldi, Consuelo Penella, Dorothybelle PoliMartina Pollastrini, Zdzislawa B. Romanowska-Duda, Beata Rutkowska, João Serôdio, Kancherla Suresh, Wiesław Szulc, Eduardo Tambussi, Marcos Yanniccari, Marek Zivcak

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

Original languageEnglish
Pages (from-to)121-158
Number of pages38
JournalPhotosynthesis Research
Volume122
Issue number2
DOIs
Publication statusPublished - 2014

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Chlorophyll
Fluorescence
fluorescence
chlorophyll
Dark Adaptation
dark adaptation
hands
Hand
methodology
chlorophyll a
Technology
Hardware

Keywords

  • Chlorophyll a fluorescence
  • Complementary techniques
  • Fluorescence imaging
  • Frequently asked questions
  • Photosynthesis
  • Plant stress monitoring

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry
  • Medicine(all)

Cite this

Kalaji, H. M., Schansker, G., Ladle, R. J., Goltsev, V., Bosa, K., Allakhverdiev, S. I., ... Zivcak, M. (2014). Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues. Photosynthesis Research, 122(2), 121-158. https://doi.org/10.1007/s11120-014-0024-6

Frequently asked questions about in vivo chlorophyll fluorescence : Practical issues. / Kalaji, Hazem M.; Schansker, G.; Ladle, Richard J.; Goltsev, Vasilij; Bosa, Karolina; Allakhverdiev, Suleyman I.; Brestic, Marian; Bussotti, Filippo; Calatayud, Angeles; Dąbrowski, Piotr; Elsheery, Nabil I.; Ferroni, Lorenzo; Guidi, Lucia; Hogewoning, Sander W.; Jajoo, Anjana; Misra, Amarendra N.; Nebauer, Sergio G.; Pancaldi, Simonetta; Penella, Consuelo; Poli, Dorothybelle; Pollastrini, Martina; Romanowska-Duda, Zdzislawa B.; Rutkowska, Beata; Serôdio, João; Suresh, Kancherla; Szulc, Wiesław; Tambussi, Eduardo; Yanniccari, Marcos; Zivcak, Marek.

In: Photosynthesis Research, Vol. 122, No. 2, 2014, p. 121-158.

Research output: Contribution to journalArticle

Kalaji, HM, Schansker, G, Ladle, RJ, Goltsev, V, Bosa, K, Allakhverdiev, SI, Brestic, M, Bussotti, F, Calatayud, A, Dąbrowski, P, Elsheery, NI, Ferroni, L, Guidi, L, Hogewoning, SW, Jajoo, A, Misra, AN, Nebauer, SG, Pancaldi, S, Penella, C, Poli, D, Pollastrini, M, Romanowska-Duda, ZB, Rutkowska, B, Serôdio, J, Suresh, K, Szulc, W, Tambussi, E, Yanniccari, M & Zivcak, M 2014, 'Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues', Photosynthesis Research, vol. 122, no. 2, pp. 121-158. https://doi.org/10.1007/s11120-014-0024-6
Kalaji, Hazem M. ; Schansker, G. ; Ladle, Richard J. ; Goltsev, Vasilij ; Bosa, Karolina ; Allakhverdiev, Suleyman I. ; Brestic, Marian ; Bussotti, Filippo ; Calatayud, Angeles ; Dąbrowski, Piotr ; Elsheery, Nabil I. ; Ferroni, Lorenzo ; Guidi, Lucia ; Hogewoning, Sander W. ; Jajoo, Anjana ; Misra, Amarendra N. ; Nebauer, Sergio G. ; Pancaldi, Simonetta ; Penella, Consuelo ; Poli, Dorothybelle ; Pollastrini, Martina ; Romanowska-Duda, Zdzislawa B. ; Rutkowska, Beata ; Serôdio, João ; Suresh, Kancherla ; Szulc, Wiesław ; Tambussi, Eduardo ; Yanniccari, Marcos ; Zivcak, Marek. / Frequently asked questions about in vivo chlorophyll fluorescence : Practical issues. In: Photosynthesis Research. 2014 ; Vol. 122, No. 2. pp. 121-158.
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AU - Goltsev, Vasilij

AU - Bosa, Karolina

AU - Allakhverdiev, Suleyman I.

AU - Brestic, Marian

AU - Bussotti, Filippo

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AU - Suresh, Kancherla

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