Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses - Short communication

Krisztián Bányai, Jelle Matthijnssens, György Szücs, Petra Forgách, Károly Erdélyi, Marc Ranst, Eleonora Lorusso, Nicola Decaro, Gabriella Elia, Vito Martella

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In rotaviruses, intragenic recombination or gene rearrangement occurs almost exclusively in the genome segments encoding for non-structural proteins. Rearranged RNA originates by mechanisms of partial sequence duplications and deletions or insertions of non-templated nucleotides. Of interest, epidemiological investigations have pointed out an unusual bias to rearrangements in genome segment 11, notably in rotavirus strains of lapine origin, as evidenced by the detection of numerous lapine strains with super-short genomic electropherotype. The sequence of the full-length genome segment 11 of two lapine strains with super-short electropherotype, LRV-4 and 3489/3, was determined and compared with rearranged and normal cognate genome segments of lapine rotaviruses. The rearranged genome segments contained head-to-tail partial duplications at the 3′ end of the main ORF encoding NSP5. Unlike the strains Alabama and B4106, intermingled stretches of non-templated sequences were not present in the accessory RNA of LRV-4 and 3489/3, while multiple deletions were mapped, suggesting the lack of functional constraints. Altogether, these findings suggest that independent rearrangement events have given origin to the various lapine strains that have super-short genome pattern.

Original languageEnglish
Pages (from-to)453-461
Number of pages9
JournalActa veterinaria Hungarica
Volume57
Issue number3
DOIs
Publication statusPublished - Sep 1 2009

Keywords

  • Deletion
  • Evolution
  • Gene duplication
  • Recombination

ASJC Scopus subject areas

  • veterinary(all)

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