Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning

Anikó Brumbauer, Göte Johansson, K. Réczey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An aqueous two-phase system based on the two polymers poly(ethylene glycol) and dextran has been used for the fractionation of cellulase enzymes present in culture liquid obtained by fermentation with Trichoderma reesei. The activities of β-glucosidase and glucanases were separated to high degree by using the two-phase systems for a counter-current distribution process in nine transfer steps. While the glucanases had high affinity to the poly(ethylene glycol) rich top phase the β-glucosidase was enriched in the dextran-containing bottom phase. Multiple counter-current distribution performed indicates the heterogeneity of β-glucosidase activities assuming at least four isoenzyme forms. One step concentration of β-glucosidase by using system with 46:1 phase volume ratio resulted in 16 times higher enzyme activity.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalBioseparation
Volume7
Issue number6
DOIs
Publication statusPublished - 1999

Fingerprint

Glucosidases
Trichoderma
Cellulase
Fractionation
Dextran
Ethylene Glycol
Liquids
Dextrans
Polyethylene glycols
Isoenzymes
Enzyme activity
Enzymes
Fermentation
Polymers

Keywords

  • β-glucosidase
  • Aqueous two-phase systems
  • Cellulases
  • Fractionation of enzymes
  • Partitioning of proteins
  • Trichoderma reesei

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry
  • Chemical Engineering(all)

Cite this

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning. / Brumbauer, Anikó; Johansson, Göte; Réczey, K.

In: Bioseparation, Vol. 7, No. 6, 1999, p. 287-295.

Research output: Contribution to journalArticle

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