Fphog1, a HOG-type MAP kinase gene, is involved in multistress response in Fusarium proliferatum

A. Ádám, Gábor Kohut, L. Hornok

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

ΔFphog1 mutants of Fusarium proliferatum obtained by targeted gene disruption of Fphog1, an orthologue of the Saccharomyces cerevisiae hog1 MAPK gene showed increased sensitivity towards different abiotic stressors including UV-irradiation, heat, salt, osmotic and hydrogen peroxide treatments. Incubation of the ΔFphog1 mutants under hyperosmotic conditions was accompanied with prolonged growth arrest, inhibition of conidial germination, morphological abnormalities and time-dependent increase of the cell death rate. The wild type Fphog1 gene, under the control of its own promoter, was able to rescue the multistress sensitivity of the mutant strain. Real time qPCR data demonstrated that under salt and sorbitol stress conditions the Fphog1 gene is not subject of transcriptional regulation. Levels of reactive oxygen species (ROS), mitochondrial membrane permeability transition, nuclear disintegration and DNA fragmentation, indicators of programmed cell death (PCD) all showed significant increases under osmotic stress conditions in the ΔFphog1 mutant in comparison to the wild type strain. These results suggest that an important function of Fphog1 is attenuating apoptotic phenotypes under salt and sorbitol stressors.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalJournal of Basic Microbiology
Volume48
Issue number3
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Fusarium
Phosphotransferases
Sorbitol
Salts
Genes
Cell Death
Osmotic Pressure
Mitochondrial Membranes
DNA Fragmentation
Germination
Hydrogen Peroxide
Saccharomyces cerevisiae
Permeability
Reactive Oxygen Species
Hot Temperature
Phenotype
Mortality
Growth

Keywords

  • Fusarium
  • HOG-type mitogen-activated protein kinase
  • Hyperosmotic stress
  • Programmed cell death
  • Salt stress

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Fphog1, a HOG-type MAP kinase gene, is involved in multistress response in Fusarium proliferatum. / Ádám, A.; Kohut, Gábor; Hornok, L.

In: Journal of Basic Microbiology, Vol. 48, No. 3, 06.2008, p. 151-159.

Research output: Contribution to journalArticle

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