Fourier transform infrared and electron paramagnetic resonance spectroscopic studies of thylakoid membranes

Zoltán Kóta, B. Szalontai, Magdolna Droppa, G. Horváth, T. Páli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Changes in both structure and dynamics of thylakoid membranes exposed to heat stress during greening of etiolated barley seedlings were studied using complementary spectroscopic techniques. FTIR spectra of thylakoid membranes were analyzed in the Amide I and the symmetric CH2 stretching vibration regions providing data on protein secondary structures and lipid acyl chain conformations, respectively. The partitioning of 2,2,6,6,- tetramethylpiperidine-1-oxyl (TEMPO) and chain dynamics of 5-(4',4'- dimethyloxazolidine-N-oxyl)stearic acid (5-SASL) spin labels in thylakoid membranes were measured using EPR spectroscopy. Both proteins and lipids responded to heat stress with major structural changes around 40°C and 50°C, at any stages of greening, which can be related to the dissociation of the light harvesting complexes and the exclusion of non-bilayer-forming lipids from the membrane, respectively. Greening was found to be a multistage process concerning both mobility, packing density and conformation of lipid fatty acyl chains as well as protein secondary structure, with correlated events during illumination.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalJournal of Molecular Structure
Volume480-481
DOIs
Publication statusPublished - May 4 1999

Fingerprint

Thylakoids
Electron Spin Resonance Spectroscopy
Fourier Analysis
Paramagnetic resonance
Secondary Protein Structure
lipids
electron paramagnetic resonance
Fourier transforms
membranes
Infrared radiation
Membranes
Lipids
Conformations
Hot Temperature
proteins
Spin Labels
Proteins
barley
Fourier Transform Infrared Spectroscopy
Hordeum

Keywords

  • FTIR
  • Greening
  • Heat stress
  • Spin label EPR
  • Thylakoid membrane structure

ASJC Scopus subject areas

  • Structural Biology
  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Fourier transform infrared and electron paramagnetic resonance spectroscopic studies of thylakoid membranes. / Kóta, Zoltán; Szalontai, B.; Droppa, Magdolna; Horváth, G.; Páli, T.

In: Journal of Molecular Structure, Vol. 480-481, 04.05.1999, p. 395-400.

Research output: Contribution to journalArticle

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