Forskolin-loaded human serum albumin nanoparticles and its biological importance

Veerababu Nagati, Sailaja Nakkka, Daniel Pushparaju Yeggoni, S. Rajagopal

Research output: Contribution to journalArticle

Abstract

In this study, forskolin-loaded human serum albumin nanoparticles (FR-HSANPs) were successfully prepared by incorporation and affinity-binding methods. FR-HSANPs were characterized by transmission electron microscope that most of them are circular in shape and size is around 340 nm. The drug loading was more than 88% and further sustained release profiles were observed as it is 77.5% in 24 h time. Additionally, the cytotoxicity results with HepG2 cells indicated that FR-HSANPs showed significantly higher cytotoxicity and lower cell viability as compared to free forskolin (FR). Furthermore, to understand the binding mechanism of human serum albumin (HSA) with forskolin resulted from fluorescence quenching as a static mechanism and the binding constant is 6.26 ± 0.1 × 10 4 M −1 , indicating a strong binding affinity. Further, association and dissociation kinetics of forskolin–HSA was calculated from surface plasmon resonance spectroscopy and the binding constant found to be K forskolin = 3.4 ± 0.24 × 10 4 M −1 and also fast dissociation was observed. Further, we used circular dichroism and molecular dynamics simulations to elucidate the possible structural changes including local conformational changes and rigidity of the residues of both HSA and HSA–forskolin complexes. Communicated by Ramaswamy H. Sarma.

Original languageEnglish
JournalJournal of Biomolecular Structure and Dynamics
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Colforsin
Serum Albumin
Nanoparticles
Surface Plasmon Resonance
Hep G2 Cells
Molecular Dynamics Simulation
Circular Dichroism
Spectrum Analysis
Cell Survival
Fluorescence
Electrons
Pharmaceutical Preparations

Keywords

  • Drug loading
  • human serum albumin nanoparticles
  • molecular dynamics simulations
  • surface plasmon resonance
  • transmission electron microscope

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Forskolin-loaded human serum albumin nanoparticles and its biological importance. / Nagati, Veerababu; Nakkka, Sailaja; Yeggoni, Daniel Pushparaju; Rajagopal, S.

In: Journal of Biomolecular Structure and Dynamics, 01.01.2019.

Research output: Contribution to journalArticle

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