Formin proteins of the DAAM subfamily play a role during axon growth

Tamás Matusek, Rita Gombos, Anita Szécsényi, Natalia Sánchez-Soriano, Ágnes Czibula, Csilla Pataki, Anita Gedai, Andreas Prokop, István Raskó, József Mihály

Research output: Contribution to journalArticle

71 Citations (Scopus)


The regulation of growth cone actin dynamics is a critical aspect of axonal growth control. Among the proteins that are directly involved in the regulation of actin dynamics, actin nucleation factors play a pivotal role by promoting the formation of novel actin filaments. However, the essential nucleation factors in developing neurons have so far not been clearly identified. Here, we show expression data, and use true loss-of-function analysis and targeted expression of activated constructs to demonstrate that the Drosophila formin DAAM plays a critical role in axonal morphogenesis. In agreement with this finding, we show that dDAAM is required for filopodia formation at axonal growth cones. Our genetic interaction, immunoprecipitation and protein localization studies argue that dDAAM acts in concert with Rac GTPases, Profilin and Enabled during axonal growth regulation. We also show that mouse Daam1 rescues the CNS defects observed in dDAAM mutant flies to a high degree, and vice versa, that Drosophila DAAM induces the formation of neurite-like protrusions when expressed in mouse P19 cells, strongly suggesting that the function of DAAM in developing neurons has been conserved during evolution.

Original languageEnglish
Pages (from-to)13310-13319
Number of pages10
JournalJournal of Neuroscience
Issue number49
Publication statusPublished - Dec 3 2008


  • Axon growth
  • Ena
  • Filopodia formation
  • Formin
  • Profilin
  • Rac
  • dDAAM

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Formin proteins of the DAAM subfamily play a role during axon growth'. Together they form a unique fingerprint.

  • Cite this

    Matusek, T., Gombos, R., Szécsényi, A., Sánchez-Soriano, N., Czibula, Á., Pataki, C., Gedai, A., Prokop, A., Raskó, I., & Mihály, J. (2008). Formin proteins of the DAAM subfamily play a role during axon growth. Journal of Neuroscience, 28(49), 13310-13319.