Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen

B. Schoket, A. Hewer, P. L. Grover, D. H. Phillips

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Adult and foetal human skin samples maintained in short-term organ culture were trained topically with solutions of coal-tar, creosote or bitumen, then DNA was isolated and analysed by 32P-post-labelling for the presence of aromatic DNA adducts. Autoradiographs of the 32P-labelled adducts resolved on polyethyleneimine-cellulose tlc sheets revealed a band of radioactivity indicative of the formation of adducts by a large number of components in these complex carcinogenic mixtures. Single doses of the materials, similar to those used to initiate tumours in experimental animals, resulted in the formation of approximately 0.3 fmol total adducts/μg DNA. The levels of adducts formed in human skin are thus similar to those formed in mouse skin after administration of doses known to be cardiogenic to the latter. The results provide direct evidence of DNA damage in human skin by materials strongly suspected of being carcinogenic to humans, and point to a method for evaluating other complex mixtures of aromatic chemicals for their potential carcinogenic hazard.

Original languageEnglish
Pages (from-to)622-626
Number of pages5
JournalInternational Journal of Cancer
Volume42
Issue number4
Publication statusPublished - 1988

Fingerprint

asphalt
Creosote
Coal Tar
DNA Adducts
Organ Culture Techniques
Skin
Complex Mixtures
Radioactivity
DNA Damage
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen. / Schoket, B.; Hewer, A.; Grover, P. L.; Phillips, D. H.

In: International Journal of Cancer, Vol. 42, No. 4, 1988, p. 622-626.

Research output: Contribution to journalArticle

@article{260546df62434739b1efa700e9bfc596,
title = "Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen",
abstract = "Adult and foetal human skin samples maintained in short-term organ culture were trained topically with solutions of coal-tar, creosote or bitumen, then DNA was isolated and analysed by 32P-post-labelling for the presence of aromatic DNA adducts. Autoradiographs of the 32P-labelled adducts resolved on polyethyleneimine-cellulose tlc sheets revealed a band of radioactivity indicative of the formation of adducts by a large number of components in these complex carcinogenic mixtures. Single doses of the materials, similar to those used to initiate tumours in experimental animals, resulted in the formation of approximately 0.3 fmol total adducts/μg DNA. The levels of adducts formed in human skin are thus similar to those formed in mouse skin after administration of doses known to be cardiogenic to the latter. The results provide direct evidence of DNA damage in human skin by materials strongly suspected of being carcinogenic to humans, and point to a method for evaluating other complex mixtures of aromatic chemicals for their potential carcinogenic hazard.",
author = "B. Schoket and A. Hewer and Grover, {P. L.} and Phillips, {D. H.}",
year = "1988",
language = "English",
volume = "42",
pages = "622--626",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen

AU - Schoket, B.

AU - Hewer, A.

AU - Grover, P. L.

AU - Phillips, D. H.

PY - 1988

Y1 - 1988

N2 - Adult and foetal human skin samples maintained in short-term organ culture were trained topically with solutions of coal-tar, creosote or bitumen, then DNA was isolated and analysed by 32P-post-labelling for the presence of aromatic DNA adducts. Autoradiographs of the 32P-labelled adducts resolved on polyethyleneimine-cellulose tlc sheets revealed a band of radioactivity indicative of the formation of adducts by a large number of components in these complex carcinogenic mixtures. Single doses of the materials, similar to those used to initiate tumours in experimental animals, resulted in the formation of approximately 0.3 fmol total adducts/μg DNA. The levels of adducts formed in human skin are thus similar to those formed in mouse skin after administration of doses known to be cardiogenic to the latter. The results provide direct evidence of DNA damage in human skin by materials strongly suspected of being carcinogenic to humans, and point to a method for evaluating other complex mixtures of aromatic chemicals for their potential carcinogenic hazard.

AB - Adult and foetal human skin samples maintained in short-term organ culture were trained topically with solutions of coal-tar, creosote or bitumen, then DNA was isolated and analysed by 32P-post-labelling for the presence of aromatic DNA adducts. Autoradiographs of the 32P-labelled adducts resolved on polyethyleneimine-cellulose tlc sheets revealed a band of radioactivity indicative of the formation of adducts by a large number of components in these complex carcinogenic mixtures. Single doses of the materials, similar to those used to initiate tumours in experimental animals, resulted in the formation of approximately 0.3 fmol total adducts/μg DNA. The levels of adducts formed in human skin are thus similar to those formed in mouse skin after administration of doses known to be cardiogenic to the latter. The results provide direct evidence of DNA damage in human skin by materials strongly suspected of being carcinogenic to humans, and point to a method for evaluating other complex mixtures of aromatic chemicals for their potential carcinogenic hazard.

UR - http://www.scopus.com/inward/record.url?scp=0023804129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023804129&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 622

EP - 626

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -