Formation and transposition of the covalently closed IS30 circle: The relation between tandem dimers and monomeric circles

János Kiss, F. Olasz

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In the present study, we demonstrate that a circular IS30 element acts as an intermediate for simple insertion. Covalently closed IS and Tn circles constructed in vitro are suitable for integration into the host genome. Minicircle integration displays all the characteristics of transpositional fusion mediated by the (IS30)2 dimer regarding target selection and target duplication. Evidence is provided for in vivo circularization of the element located either on plasmids or on the genome. It is shown that circle formation can occur through alternative pathways. One of them is excision of IS30 from a hot spot via joining the IRs. This reaction resembles the site-specific dimerization that leads to (IS30)2 establishment. The other process is the dissolution of (IS30)2 dimer, when the element is excised from an IR-IR joint. These pathways differ basically in the fate of the donor replicon: only dimer dissolution gives rise to resealed donor backbone. Analysis of minicircles and the rearranged donor replicons led us to propose a molecular model that can account for differences between the circle-generating processes. Our focus was to the dissolution of IR-IR joints located on the host genome, because these events promoted extensive genomic rearrangements and accompanied mini-circle formation. The results present the possibility of host genome reorganization by IS30-like transposition.

Original languageEnglish
Pages (from-to)37-52
Number of pages16
JournalMolecular Microbiology
Volume34
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Genome
Replicon
Joints
Molecular Models
Dimerization
Plasmids
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Formation and transposition of the covalently closed IS30 circle : The relation between tandem dimers and monomeric circles. / Kiss, János; Olasz, F.

In: Molecular Microbiology, Vol. 34, No. 1, 1999, p. 37-52.

Research output: Contribution to journalArticle

@article{5c82af83ae41493b8c6d6f87ba6196a3,
title = "Formation and transposition of the covalently closed IS30 circle: The relation between tandem dimers and monomeric circles",
abstract = "In the present study, we demonstrate that a circular IS30 element acts as an intermediate for simple insertion. Covalently closed IS and Tn circles constructed in vitro are suitable for integration into the host genome. Minicircle integration displays all the characteristics of transpositional fusion mediated by the (IS30)2 dimer regarding target selection and target duplication. Evidence is provided for in vivo circularization of the element located either on plasmids or on the genome. It is shown that circle formation can occur through alternative pathways. One of them is excision of IS30 from a hot spot via joining the IRs. This reaction resembles the site-specific dimerization that leads to (IS30)2 establishment. The other process is the dissolution of (IS30)2 dimer, when the element is excised from an IR-IR joint. These pathways differ basically in the fate of the donor replicon: only dimer dissolution gives rise to resealed donor backbone. Analysis of minicircles and the rearranged donor replicons led us to propose a molecular model that can account for differences between the circle-generating processes. Our focus was to the dissolution of IR-IR joints located on the host genome, because these events promoted extensive genomic rearrangements and accompanied mini-circle formation. The results present the possibility of host genome reorganization by IS30-like transposition.",
author = "J{\'a}nos Kiss and F. Olasz",
year = "1999",
doi = "10.1046/j.1365-2958.1999.01567.x",
language = "English",
volume = "34",
pages = "37--52",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Formation and transposition of the covalently closed IS30 circle

T2 - The relation between tandem dimers and monomeric circles

AU - Kiss, János

AU - Olasz, F.

PY - 1999

Y1 - 1999

N2 - In the present study, we demonstrate that a circular IS30 element acts as an intermediate for simple insertion. Covalently closed IS and Tn circles constructed in vitro are suitable for integration into the host genome. Minicircle integration displays all the characteristics of transpositional fusion mediated by the (IS30)2 dimer regarding target selection and target duplication. Evidence is provided for in vivo circularization of the element located either on plasmids or on the genome. It is shown that circle formation can occur through alternative pathways. One of them is excision of IS30 from a hot spot via joining the IRs. This reaction resembles the site-specific dimerization that leads to (IS30)2 establishment. The other process is the dissolution of (IS30)2 dimer, when the element is excised from an IR-IR joint. These pathways differ basically in the fate of the donor replicon: only dimer dissolution gives rise to resealed donor backbone. Analysis of minicircles and the rearranged donor replicons led us to propose a molecular model that can account for differences between the circle-generating processes. Our focus was to the dissolution of IR-IR joints located on the host genome, because these events promoted extensive genomic rearrangements and accompanied mini-circle formation. The results present the possibility of host genome reorganization by IS30-like transposition.

AB - In the present study, we demonstrate that a circular IS30 element acts as an intermediate for simple insertion. Covalently closed IS and Tn circles constructed in vitro are suitable for integration into the host genome. Minicircle integration displays all the characteristics of transpositional fusion mediated by the (IS30)2 dimer regarding target selection and target duplication. Evidence is provided for in vivo circularization of the element located either on plasmids or on the genome. It is shown that circle formation can occur through alternative pathways. One of them is excision of IS30 from a hot spot via joining the IRs. This reaction resembles the site-specific dimerization that leads to (IS30)2 establishment. The other process is the dissolution of (IS30)2 dimer, when the element is excised from an IR-IR joint. These pathways differ basically in the fate of the donor replicon: only dimer dissolution gives rise to resealed donor backbone. Analysis of minicircles and the rearranged donor replicons led us to propose a molecular model that can account for differences between the circle-generating processes. Our focus was to the dissolution of IR-IR joints located on the host genome, because these events promoted extensive genomic rearrangements and accompanied mini-circle formation. The results present the possibility of host genome reorganization by IS30-like transposition.

UR - http://www.scopus.com/inward/record.url?scp=0032821157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032821157&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2958.1999.01567.x

DO - 10.1046/j.1365-2958.1999.01567.x

M3 - Article

C2 - 10540284

AN - SCOPUS:0032821157

VL - 34

SP - 37

EP - 52

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 1

ER -