Formalin attenuates the stress-induced increase in plasma epinephrine levels

B. Mravec, I. Bodnár, G. Uhereczky, G. M. Nagy, R. Kvetnansky, M. Palkóvits

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Subcutaneous (s.c.) injection of formalin into rats is frequently used as a painful stressor that produces a three-phase nociceptive response. We have shown previously that s.c. administered formalin (0.2 ml of 4% solution per 100 g body weight) unexpectedly attenuated the increase of plasma epinephrine levels in rats exposed to exteroceptive stressors (handling, immobilisation). To clarify the mechanism(s) responsible for this phenomenon, the effect of formalin applications on epinephrine plasma levels was investigated in various experimental conditions. Subcutaneous application of formalin combined with exposures of animals to an interoceptive stressor, insulin-induced hypoglycaemia, significantly attenuated the stress-induced increase in plasma epinephrine levels, whereas plasma norepinephrine levels remained highly elevated. Moreover, administration of formalin to unstressed animals also manifested signs of an attenuated epinephrine secretion. Interestingly, intraperitoneal administration of formalin did not reduce the elevated levels of plasma epinephrine. We suggest that formalin attenuates epinephrine secretion from the adrenal medulla most probably via irritation of s.c. somatosensory receptors. We hypothesise that the irritation of the primary sensory afferents fibres might reduce the activity of the sympathetic preganglionic neurones innervating adrenal medullary chromaffin cells. Further investigations are required to establish whether the observed reduction of epinephrine secretion from the adrenal medulla is controlled by either spinal or supraspinal neuronal circuits.

Original languageEnglish
Pages (from-to)727-732
Number of pages6
JournalJournal of Neuroendocrinology
Volume17
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Formaldehyde
Epinephrine
Adrenal Medulla
Chromaffin Cells
Subcutaneous Injections
Hypoglycemia
Immobilization
Norepinephrine
Body Weight
Insulin
Neurons

Keywords

  • 1-methyl-3
  • 4-dihydroisoquinoline
  • Epinephrine
  • Formalin
  • Hypoglycaemia
  • Immobilisation
  • Norepinephrine

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Formalin attenuates the stress-induced increase in plasma epinephrine levels. / Mravec, B.; Bodnár, I.; Uhereczky, G.; Nagy, G. M.; Kvetnansky, R.; Palkóvits, M.

In: Journal of Neuroendocrinology, Vol. 17, No. 11, 11.2005, p. 727-732.

Research output: Contribution to journalArticle

Mravec, B. ; Bodnár, I. ; Uhereczky, G. ; Nagy, G. M. ; Kvetnansky, R. ; Palkóvits, M. / Formalin attenuates the stress-induced increase in plasma epinephrine levels. In: Journal of Neuroendocrinology. 2005 ; Vol. 17, No. 11. pp. 727-732.
@article{b19558e7f01241818515c51a2ae8d39a,
title = "Formalin attenuates the stress-induced increase in plasma epinephrine levels",
abstract = "Subcutaneous (s.c.) injection of formalin into rats is frequently used as a painful stressor that produces a three-phase nociceptive response. We have shown previously that s.c. administered formalin (0.2 ml of 4{\%} solution per 100 g body weight) unexpectedly attenuated the increase of plasma epinephrine levels in rats exposed to exteroceptive stressors (handling, immobilisation). To clarify the mechanism(s) responsible for this phenomenon, the effect of formalin applications on epinephrine plasma levels was investigated in various experimental conditions. Subcutaneous application of formalin combined with exposures of animals to an interoceptive stressor, insulin-induced hypoglycaemia, significantly attenuated the stress-induced increase in plasma epinephrine levels, whereas plasma norepinephrine levels remained highly elevated. Moreover, administration of formalin to unstressed animals also manifested signs of an attenuated epinephrine secretion. Interestingly, intraperitoneal administration of formalin did not reduce the elevated levels of plasma epinephrine. We suggest that formalin attenuates epinephrine secretion from the adrenal medulla most probably via irritation of s.c. somatosensory receptors. We hypothesise that the irritation of the primary sensory afferents fibres might reduce the activity of the sympathetic preganglionic neurones innervating adrenal medullary chromaffin cells. Further investigations are required to establish whether the observed reduction of epinephrine secretion from the adrenal medulla is controlled by either spinal or supraspinal neuronal circuits.",
keywords = "1-methyl-3, 4-dihydroisoquinoline, Epinephrine, Formalin, Hypoglycaemia, Immobilisation, Norepinephrine",
author = "B. Mravec and I. Bodn{\'a}r and G. Uhereczky and Nagy, {G. M.} and R. Kvetnansky and M. Palk{\'o}vits",
year = "2005",
month = "11",
doi = "10.1111/j.1365-2826.2005.01365.x",
language = "English",
volume = "17",
pages = "727--732",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Formalin attenuates the stress-induced increase in plasma epinephrine levels

AU - Mravec, B.

AU - Bodnár, I.

AU - Uhereczky, G.

AU - Nagy, G. M.

AU - Kvetnansky, R.

AU - Palkóvits, M.

PY - 2005/11

Y1 - 2005/11

N2 - Subcutaneous (s.c.) injection of formalin into rats is frequently used as a painful stressor that produces a three-phase nociceptive response. We have shown previously that s.c. administered formalin (0.2 ml of 4% solution per 100 g body weight) unexpectedly attenuated the increase of plasma epinephrine levels in rats exposed to exteroceptive stressors (handling, immobilisation). To clarify the mechanism(s) responsible for this phenomenon, the effect of formalin applications on epinephrine plasma levels was investigated in various experimental conditions. Subcutaneous application of formalin combined with exposures of animals to an interoceptive stressor, insulin-induced hypoglycaemia, significantly attenuated the stress-induced increase in plasma epinephrine levels, whereas plasma norepinephrine levels remained highly elevated. Moreover, administration of formalin to unstressed animals also manifested signs of an attenuated epinephrine secretion. Interestingly, intraperitoneal administration of formalin did not reduce the elevated levels of plasma epinephrine. We suggest that formalin attenuates epinephrine secretion from the adrenal medulla most probably via irritation of s.c. somatosensory receptors. We hypothesise that the irritation of the primary sensory afferents fibres might reduce the activity of the sympathetic preganglionic neurones innervating adrenal medullary chromaffin cells. Further investigations are required to establish whether the observed reduction of epinephrine secretion from the adrenal medulla is controlled by either spinal or supraspinal neuronal circuits.

AB - Subcutaneous (s.c.) injection of formalin into rats is frequently used as a painful stressor that produces a three-phase nociceptive response. We have shown previously that s.c. administered formalin (0.2 ml of 4% solution per 100 g body weight) unexpectedly attenuated the increase of plasma epinephrine levels in rats exposed to exteroceptive stressors (handling, immobilisation). To clarify the mechanism(s) responsible for this phenomenon, the effect of formalin applications on epinephrine plasma levels was investigated in various experimental conditions. Subcutaneous application of formalin combined with exposures of animals to an interoceptive stressor, insulin-induced hypoglycaemia, significantly attenuated the stress-induced increase in plasma epinephrine levels, whereas plasma norepinephrine levels remained highly elevated. Moreover, administration of formalin to unstressed animals also manifested signs of an attenuated epinephrine secretion. Interestingly, intraperitoneal administration of formalin did not reduce the elevated levels of plasma epinephrine. We suggest that formalin attenuates epinephrine secretion from the adrenal medulla most probably via irritation of s.c. somatosensory receptors. We hypothesise that the irritation of the primary sensory afferents fibres might reduce the activity of the sympathetic preganglionic neurones innervating adrenal medullary chromaffin cells. Further investigations are required to establish whether the observed reduction of epinephrine secretion from the adrenal medulla is controlled by either spinal or supraspinal neuronal circuits.

KW - 1-methyl-3

KW - 4-dihydroisoquinoline

KW - Epinephrine

KW - Formalin

KW - Hypoglycaemia

KW - Immobilisation

KW - Norepinephrine

UR - http://www.scopus.com/inward/record.url?scp=27644586443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644586443&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2826.2005.01365.x

DO - 10.1111/j.1365-2826.2005.01365.x

M3 - Article

C2 - 16219001

AN - SCOPUS:27644586443

VL - 17

SP - 727

EP - 732

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 11

ER -