Formaldehyde and some fully N-methylated substances in boar seminal fluids: Short Communication

M. Szilágyi, Zs I. Németh, L. Albert, P. Sarlós, E. Tyihák

Research output: Contribution to journalArticle

Abstract

On the basis of recent observations it is supposed that seminal fluids may contain - mainly in hydroxymethyl groups - formaldehyde (HCHO) and quaternary ammonium compounds as potential HCHO generators, therefore, preliminary investigations were carried out for the identification of these compounds in pig seminal fluids using OPLC, HPLC and MALDI MS techniques. The fresh pig seminal fluid was frozen in liquid nitrogen, powdered and aliquots (0.25 g) were treated with 0.7 ml ethanolic dimedone solution. The suspension was centrifuged and the clear supernatant was used for analysis by OPLC or after dilution with HPLC or MALDI MS technique. After OPLC separation of formaldemethone the fully N-methylated compounds which are stayed on the start point were separated by OPLC using an other eluent system. It has been established that the HCHO is really a normal component of the pig seminal fluid, as well. It can be isolated and identified in dimedone adduct form. The measurable amount of HCHO depended on the concentration applied of dimedone. According to OPLC and MALDI MS investigations L-carnitine is the main quaternary ammonium compound in pig seminal fluid which can generate a protection of the sperm cells against environmental and other influences. Considerable differences have been found among individuals concerning concentrations of quaternary ammonium compounds in the seminal fluid of pigs.

Original languageEnglish
Pages (from-to)133-136
Number of pages4
JournalActa biologica Hungarica
Volume57
Issue number1
DOIs
Publication statusPublished - Jul 21 2006

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Keywords

  • Carnitine
  • Formaldehyde
  • Pig
  • Seminal fluid
  • Trigonelline

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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