Forced-flow planar liquid chromatographic techniques (after twenty-two years)

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In the twenty-two years since a similar review in the first issue of this journal there has been much progress in forced-flow planar liquid chromatography (FFPLC). Innovative developments have resulted in extremely diverse new technical solutions. This review briefly summarizes techniques which are already in use as a result of this progress (overpressured-layer chromatography, OPLC, and rotation planar chromatography, RPC) and the future potential of other FFPLC techniques, for example electrochromatographic techniques and shear-driven chromatography (SDC). It seems that efforts in FFPLC provide real possibilities of efficient analytical and preparative separations of different complex mixtures exploiting, with increasing success, the objective advantages of planar layer chromatography. This review summarizes, first, the unique opportunities resulting from modern biological detection on the adsorbent layer (BioArena) and some recent results obtained. These results include-among others-the indirect detection of small key molecules (e.g. formaldehyde, HCHO, and ozone, O 3) in chromatographic spots. Practical applications are mainly in-vivo investigations-already without chromatographic separations-with special emphasis on disease resistance and cell proliferation. It seems, however, that in the future BioArena-guided in-vivo investigation will enable characterization of biologically active substances.

Original languageEnglish
Pages (from-to)382-395
Number of pages14
JournalJournal of Planar Chromatography - Modern TLC
Volume23
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

Fingerprint

Chromatography
Liquid chromatography
Liquid Chromatography
Liquids
Adsorbents
Disease Resistance
Ozone
Cell proliferation
Complex Mixtures
Formaldehyde
Cell Proliferation
Molecules

Keywords

  • BioArena
  • Direct bioautography
  • Forced flow
  • Forced-flow liquid chromatographic techniques
  • Layer chromatography
  • Ozone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Forced-flow planar liquid chromatographic techniques (after twenty-two years). / Tyihák, E.; Mincsovics, E.

In: Journal of Planar Chromatography - Modern TLC, Vol. 23, No. 6, 01.12.2010, p. 382-395.

Research output: Contribution to journalArticle

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