Column and layer liquid chromatographic techniques as supplementary techniques due to their arrangements have always been characteristically developed in constant mutual interaction. Hence it is not surprising that the intensive development of forcedflow column liquid chromatography (high-performance liquid chromatography, HPLC) as originally potential planar layer version of HPLC entailed the need for the fundamental renewal of the most popular planar layer liquid chromatographic technique, TLC. HPTLC is based on the use of fine particle chromatoplates with narrow particle size distribution of adsorbent and is carried out with capillary-driven separation and sophisticated instrumentation . However, the greatly increased developing time on a fine particle adsorbent layer (quadratic law exists concerning the front migration distance against the time ) made it necessary to employ forced mobile phase flow generating or at least approaching the optimum linear velocity to yield the highest efficiency of separation allowed by the layer adsorbent bed characteristic.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Agricultural and Biological Sciences(all)