Forced-Flow Development in Overpressured Layer Chromatography

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

This chapter briefly summarizes the progress of overpressured layer chromatography (OPLC) technique from 1979 to the end of 2013. Main technical and methodological solutions, moreover, important theoretical aspects as mobile phase movement and efficiency characteristics are also demonstrated. Transfusion, infusion, and infusion-transfusion operations as well as off-line and online processes are explained showing the flexibility of the OPLC systems. The multi-inlet and multi-outlet OPLC system give the possibility of online parallel sample separations by means of the flowing eluent wall and aliquot detection processes. OPLC separation hyphenated in off-line and/or online modes with other chromatographic and spectroscopic systems illustrate the potential in the field of analysis, isolation, and structure elucidation as well. The off-line combination of OPLC separation with direct bioautography and the activity-guided online isolation show a great possibility to find new biologically active compounds, moreover, the BioArena system helps to study the interaction of microbes with endogenous and/or exogenous cofactor molecules in the adsorbent layer bed.

Original languageEnglish
Title of host publicationInstrumental Thin-Layer Chromatography
PublisherElsevier Inc.
Pages107-133
Number of pages27
ISBN (Print)9780124172845, 9780124172234
DOIs
Publication statusPublished - Sep 24 2014

Fingerprint

Chromatography
Adsorbents
Molecules

Keywords

  • Aliquot online detection
  • BioArena
  • Bioautography
  • Flowing eluent wall
  • Off-line OPLC
  • Online OPLC
  • Overpressured layer chromatography (OPLC)
  • Plate height
  • Plate number
  • Resolution
  • Spot capacity

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Forced-Flow Development in Overpressured Layer Chromatography. / Mincsovics, E.; Móricz, A.; Tyihák, E.

Instrumental Thin-Layer Chromatography. Elsevier Inc., 2014. p. 107-133.

Research output: Chapter in Book/Report/Conference proceedingChapter

Mincsovics, E. ; Móricz, A. ; Tyihák, E. / Forced-Flow Development in Overpressured Layer Chromatography. Instrumental Thin-Layer Chromatography. Elsevier Inc., 2014. pp. 107-133
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