Follicular cells of tonsils metabolise more deoxycytidine than other cell populations

Lóránt Horváth, M. Sasvári, Tatjana Spasokukotskaja, Ferenc Antoni, M. Staub

Research output: Contribution to journalArticle

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Abstract

Nine subpopulations of tonsillar lymphocytes and the unseparated cells were compared in their utilization of exogenous deoxycytidine ([5-3H]CdR) and thymidine ([3H]TdR). Uptake phosphorylation and incorporation of labeled precursors were determined in B and T lymphocytes, in low density (LD; enriched in S phase cells)and in high density (HD; enriched in G0/G1 phase cells) cell fractions as well as in LD and HD subfractions of B and T lymphocytes, and in cells isolated from follicles of tonsils. As expected, LD cells and B lymphocytes were more active in TdR incorporation than HD cells and T lymphocytes. However, the ratio of [5-3H]CdR to [3H]TdR in their total phosphorylation and incorporation into DNA was much lower than expected value of 1: about 0.5 for total phosphorylation and about 0.3 for incorporation in all subpopulations, except for the follicular cells where these ratios were 1.0 and 0.7, respectively. These results show that the relative utilization of the two pyrimidine deoxyribonucleoside precursors varies among different lymphocyte subpopulations. However, this variation is not due to the different rate of DNA synthesis; rather,it depends on the differentiation stage of lymphocytes occurring in the germinal center of the follicles.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalImmunology Letters
Volume22
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Deoxycytidine
Palatine Tonsil
Population
B-Lymphocytes
Lymphocyte Subsets
Phosphorylation
T-Lymphocytes
Deoxyribonucleosides
Cell Cycle Resting Phase
Germinal Center
DNA
G1 Phase
S Phase
Thymidine
Lymphocytes

Keywords

  • Deoxycytidine
  • Follicular cells
  • Human
  • Salvage
  • Thymidine
  • Tonsil

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Follicular cells of tonsils metabolise more deoxycytidine than other cell populations. / Horváth, Lóránt; Sasvári, M.; Spasokukotskaja, Tatjana; Antoni, Ferenc; Staub, M.

In: Immunology Letters, Vol. 22, No. 2, 1989, p. 161-166.

Research output: Contribution to journalArticle

Horváth, Lóránt ; Sasvári, M. ; Spasokukotskaja, Tatjana ; Antoni, Ferenc ; Staub, M. / Follicular cells of tonsils metabolise more deoxycytidine than other cell populations. In: Immunology Letters. 1989 ; Vol. 22, No. 2. pp. 161-166.
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