Fluorescent probes and live imaging of plant cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescent probes are used in almost all areas of plant research ranging from molecular biology to ecophysiology studies. Development of novel fluorochromes and fluorescent proteins in combination with advanced microscopy techniques allow us to analyze cells, tissues, organs and whole plants in great detail. Live fluorescence microscopy imaging of plants is of particular importance for ecophysiology studies where complex interactions of plants and their environment need to be understood at molecular, cellular and organismal level. Here we present an overview of fluorescent probes and live cell microscopy setup for plants and provide a detailed protocol for fluorescent live-dead viability assay using fluorescein diacetate and propidium iodide fluorescent dyes.

Original languageEnglish
Title of host publicationAdvances in Plant Ecophysiology Techniques
PublisherSpringer International Publishing
Pages241-251
Number of pages11
ISBN (Electronic)9783319932330
ISBN (Print)9783319932323
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Plant Cells
Fluorescent Dyes
Microscopic examination
probe
image analysis
Imaging techniques
Molecular biology
Fluorescence microscopy
microscopy
ecophysiology
fluorescent dyes
Assays
Dyes
cells
Tissue
Microscopy
Proteins
Propidium
Optical Imaging
iodide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)

Cite this

Fodor, E., & Ayaydin, F. (2018). Fluorescent probes and live imaging of plant cells. In Advances in Plant Ecophysiology Techniques (pp. 241-251). Springer International Publishing. https://doi.org/10.1007/978-3-319-93233-0_14

Fluorescent probes and live imaging of plant cells. / Fodor, E.; Ayaydin, F.

Advances in Plant Ecophysiology Techniques. Springer International Publishing, 2018. p. 241-251.

Research output: Chapter in Book/Report/Conference proceedingChapter

Fodor, E & Ayaydin, F 2018, Fluorescent probes and live imaging of plant cells. in Advances in Plant Ecophysiology Techniques. Springer International Publishing, pp. 241-251. https://doi.org/10.1007/978-3-319-93233-0_14
Fodor E, Ayaydin F. Fluorescent probes and live imaging of plant cells. In Advances in Plant Ecophysiology Techniques. Springer International Publishing. 2018. p. 241-251 https://doi.org/10.1007/978-3-319-93233-0_14
Fodor, E. ; Ayaydin, F. / Fluorescent probes and live imaging of plant cells. Advances in Plant Ecophysiology Techniques. Springer International Publishing, 2018. pp. 241-251
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