Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species

É Szakács, M. Molnár-Láng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC)5. The pSc119.2 probe gave hybridization signals different from that of the rye 'Petkus' on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalCereal Research Communications
Volume36
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Secale cereale
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
rye
Chromosomes
genetic polymorphism
chromosomes
DNA probes
cultivars
provenance
hybridization
DNA Probes
genotype
Genotype
Secale

Keywords

  • 1BL/1RS translocation
  • Fluorescent in situ hybridization (FISH)
  • Polymorphism
  • Secale cereale

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Physiology

Cite this

Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species. / Szakács, É; Molnár-Láng, M.

In: Cereal Research Communications, Vol. 36, No. 2, 2008, p. 247-255.

Research output: Contribution to journalArticle

@article{851b2246632f495bbe90afe76b5b0ce1,
title = "Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species",
abstract = "The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC)5. The pSc119.2 probe gave hybridization signals different from that of the rye 'Petkus' on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.",
keywords = "1BL/1RS translocation, Fluorescent in situ hybridization (FISH), Polymorphism, Secale cereale",
author = "{\'E} Szak{\'a}cs and M. Moln{\'a}r-L{\'a}ng",
year = "2008",
doi = "10.1556/CRC.36.2008.2.5",
language = "English",
volume = "36",
pages = "247--255",
journal = "Cereal Research Communications",
issn = "0133-3720",
publisher = "Cereal Research Non-Profit Company",
number = "2",

}

TY - JOUR

T1 - Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species

AU - Szakács, É

AU - Molnár-Láng, M.

PY - 2008

Y1 - 2008

N2 - The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC)5. The pSc119.2 probe gave hybridization signals different from that of the rye 'Petkus' on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.

AB - The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC)5. The pSc119.2 probe gave hybridization signals different from that of the rye 'Petkus' on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.

KW - 1BL/1RS translocation

KW - Fluorescent in situ hybridization (FISH)

KW - Polymorphism

KW - Secale cereale

UR - http://www.scopus.com/inward/record.url?scp=52649173741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52649173741&partnerID=8YFLogxK

U2 - 10.1556/CRC.36.2008.2.5

DO - 10.1556/CRC.36.2008.2.5

M3 - Article

VL - 36

SP - 247

EP - 255

JO - Cereal Research Communications

JF - Cereal Research Communications

SN - 0133-3720

IS - 2

ER -