Fluorescent derivatives of bovine neurotensin 8-13 fragment

P. Kele, Eszter Illyés, G. Mező, Margit Dóda, Marianna Mák, A. Kaposi, Ferenc Sebestyén

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fluorescent derivatives of bovine neurotensin 8-13 fragment were prepared. For N-terminal labelling, 4-[7-hydroxycoumaryl]acetic acid (Hca), 4-[7-methoxycoumaryl]acetic acid (Mca) and 2-amino-3-[4-[7-methoxycoumaryl]]propionic acid (Amp) were used while the C-terminus of the peptide chain was elongated with Amp. The fluorescence excitation and emission spectra of the peptide derivatives were studied. Hca- and Mca/Amp-derivatives were easily distinguishable because of the 60 nm shift of their emission maxima. Compared with the natural sequence, the presence of an N-terminal label did not influence the biological potency in a longitudinal muscle strip of guinea-pig ileum, while labelling at the C-terminus considerably reduced the activity of the peptide.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalInternational Journal of Peptide Research and Therapeutics
Volume6
Issue number4
Publication statusPublished - 1999

Fingerprint

neurotensin (8-13)
Acetic acid
Acetic Acid
Peptides
Derivatives
Labeling
Propionic acid
Ileum
Muscle
Labels
Guinea Pigs
Fluorescence
Muscles
Neurotensin

Keywords

  • Coumarine derivatives
  • Fluorescence spectra
  • Fluorescent labels
  • Fluorescent peptides
  • Neurotensin 8-13 fragment
  • Peptide synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Bioengineering
  • Molecular Medicine
  • Drug Discovery
  • Analytical Chemistry

Cite this

Fluorescent derivatives of bovine neurotensin 8-13 fragment. / Kele, P.; Illyés, Eszter; Mező, G.; Dóda, Margit; Mák, Marianna; Kaposi, A.; Sebestyén, Ferenc.

In: International Journal of Peptide Research and Therapeutics, Vol. 6, No. 4, 1999, p. 235-238.

Research output: Contribution to journalArticle

Kele, P. ; Illyés, Eszter ; Mező, G. ; Dóda, Margit ; Mák, Marianna ; Kaposi, A. ; Sebestyén, Ferenc. / Fluorescent derivatives of bovine neurotensin 8-13 fragment. In: International Journal of Peptide Research and Therapeutics. 1999 ; Vol. 6, No. 4. pp. 235-238.
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AU - Kaposi, A.

AU - Sebestyén, Ferenc

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