Fluorescence Spectroscopy of Iron-Deficient Plants

B. Böddi, Edit Cseh, F. Láng

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8 Citations (Scopus)


Iron deficient cucumber (Cucumis sativus L.) plants and such differently supplied with iron were studied by low-temperature fluorescence emission and excitation spectroscopy. Iron deficiency caused a relative decrease and a blue-shift of the 733 nm emission and a decrease or disappearance of the 700 and 707-710 nm excitation bands which are considered to belong to chlorophyll forms of PSI. The process of iron uptake was observed after readdition of iron to previously iron-stressed plants. Iron-deficient plants (with green cotyledon) were treated with the chelator EDTA which treatment resulted in a small increase of the chlorophyll content but the fluorescence spectra of these plants differed from those of plants supplied with iron: they had blue-shifted maxima. The data presented show that fluorescence spectroscopy is a very sensitive and suitable method for studying iron deficiency, observing — indirectly — iron uptake and its utilization in plants.

Original languageEnglish
Pages (from-to)451-461
Number of pages11
JournalJournal of Plant Physiology
Issue number5
Publication statusPublished - Jan 1 1985


  • Cucumis sativus L.
  • chelator(EDTA)-effect
  • chlorophyll biosynthesis
  • chlorophyll form
  • fluorescence spectroscopy
  • iron deficiency chlorosis

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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