Fluorescence polarization spectra of granal and stromal membranes treated with linolenic acid. Orientation of the Photosystem I core complex within the membrane

Tatiana Szitó, L. Zimányi, Ágnes Faludi-Dániel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Polarized fluorescence spectra and fluorescence polarization ratios were compared in aligned isolated intact thylakoids and in granal and stromal membranes, without and after linolenic acid treatment at liquid N2 temperature in squeezed polyacrylamide gel. Separation of granal membranes from stromal membranes allowed an improved alignment of the membranes as compared to isolated intact thylakoids. As a result, a higher anisotropy of fluorescence was measured with fragments than with chloroplasts. Incorporation of linolenic acid into the membranes affected the energy migration between the complexes, and induced changes in the orientation of the complexes within the membranes, as shown by a reduced fluorescence intensity and decreasing values (but still larger than 1) of fluorescence polarization ratios at longer wavelengths. In order to interpret these changes in the fluorescence polarization ratios, model calculations were carried out, the following parameters being taken into account: the direction of the absorption and emission dipoles in the complex, the orientation of the complex in the membrane, and the fluctuation of the orientation. Calculated values of the fluorescence polarization ratio changed in a similar manner as those observed experimentally. The character of the changes of the fluorescence polarization ratio suggests a picture of the orientation of the complexes within the membranes.

Original languageEnglish
Pages (from-to)428-436
Number of pages9
JournalBBA - Bioenergetics
Volume808
Issue number3
DOIs
Publication statusPublished - Aug 7 1985

Fingerprint

Photosystem I Protein Complex
Fluorescence Polarization
alpha-Linolenic Acid
Fluorescence
Polarization
Membranes
Thylakoids
Chloroplasts
Anisotropy
Wavelength
Temperature
Liquids

Keywords

  • (Maize chloroplast)
  • Fluorescence polarization
  • Linolenic acid
  • Photosystem I
  • Pigment orientation
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biophysics

Cite this

Fluorescence polarization spectra of granal and stromal membranes treated with linolenic acid. Orientation of the Photosystem I core complex within the membrane. / Szitó, Tatiana; Zimányi, L.; Faludi-Dániel, Ágnes.

In: BBA - Bioenergetics, Vol. 808, No. 3, 07.08.1985, p. 428-436.

Research output: Contribution to journalArticle

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