Fluorescence line-narrowing spectra of Zn-cytochrome c. Temperature dependence

Henryk Koloczek, Judit Fidy, Jane M. Vanderkooi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Fluorescence site selection spectra of Zn-substituted cytochrome c and Zn-mesoporphyrin were measured as a function of excitation energy and temperature within 5-60 K. The emission energies determined for Zn-cytochrome c were comparable with those of Zn-mesoporphyrin and mesoporphyrin in polar glassy matrices and with resonance Raman spectra of heme proteins. In the site selection spectra of both Zn-cytochrome c and Zn-mesoporphyrin in polar organic glass an increase in the intensity of zero phonon emission lines was observed with an increase of temperature between 5 and 30 K. The irregular spectral changes were interpreted on the basis of the temperature dependence of the linewidth as well as possible hole-burning phenomena.

Original languageEnglish
Pages (from-to)4388-4394
Number of pages7
JournalThe Journal of Chemical Physics
Volume87
Issue number8
DOIs
Publication statusPublished - Jan 1 1987

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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