Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803

Tünde Tóth, Volha V. Chukhutsina, Sashka B. Krumova, Z. Gombos, Herbert van Amerongen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The FLIM (fluorescence lifetime imaging microscopy) technique allows picosecond fluorescence measurements at the level of the individual cell. Using this technique we were able to observe heterogeneity of cyanobacterial cells in a culture grown under controlled conditions and we were able to resolve structural variations within individual cells. It can be concluded that on the one hand the inhomogeneous distribution of photosynthetic pigments within the cell leads to variation of the fluorescence intensity, whereas on the other hand it is impossible to detect variation in the relative amounts of photosystem I and II throughout the cell. Different Synechocystis sp. PCC 6803 strain lines were compared to each other and differences were observed in the average fluorescence lifetimes obtained for individual cells of the various cell lines. The differences can be traced back to variable efficiency of excitation energy transfer from the phycobilisome antenna to the photosystems. We could successfully demonstrate that there is heterogeneity inside individual cells, within individual cultures, and between various wild-type cell lines.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages139-142
Number of pages4
DOIs
Publication statusPublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Fingerprint

Microscopic examination
Fluorescence
Imaging techniques
Cells
Photosystem I Protein Complex
Photosystem II Protein Complex
Excitation energy
Cell culture
Pigments
Energy transfer
Antennas

Keywords

  • Cyanobacteria
  • Excitation energy transfer
  • FLIM
  • Light harvesting
  • Photosystem

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)
  • General

Cite this

Tóth, T., Chukhutsina, V. V., Krumova, S. B., Gombos, Z., & van Amerongen, H. (2013). Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803. In Advanced Topics in Science and Technology in China (pp. 139-142). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_30

Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803. / Tóth, Tünde; Chukhutsina, Volha V.; Krumova, Sashka B.; Gombos, Z.; van Amerongen, Herbert.

Advanced Topics in Science and Technology in China. Springer, 2013. p. 139-142 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book/Report/Conference proceedingChapter

Tóth, T, Chukhutsina, VV, Krumova, SB, Gombos, Z & van Amerongen, H 2013, Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803. in Advanced Topics in Science and Technology in China. Advanced Topics in Science and Technology in China, Springer, pp. 139-142. https://doi.org/10.1007/978-3-642-32034-7_30
Tóth T, Chukhutsina VV, Krumova SB, Gombos Z, van Amerongen H. Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803. In Advanced Topics in Science and Technology in China. Springer. 2013. p. 139-142. (Advanced Topics in Science and Technology in China). https://doi.org/10.1007/978-3-642-32034-7_30
Tóth, Tünde ; Chukhutsina, Volha V. ; Krumova, Sashka B. ; Gombos, Z. ; van Amerongen, Herbert. / Fluorescence lifetime imaging microscopy of synechocystis WT cells — variation in photosynthetic performance of individual cells in various strains of sp. PCC 6803. Advanced Topics in Science and Technology in China. Springer, 2013. pp. 139-142 (Advanced Topics in Science and Technology in China).
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