Fluorescence induction reveals organization of antenna and reaction center in photosynthetic bacteria

László Gerencsér, Péter Maróti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The photochemical phase of the bacteriochlorophyll fluorescence induction generated by rectangular shape of laser diode illumination was measured in different organization levels (whole cell, chromatophore and isolated reaction center protein) of carotenoidless mutant of purple photosynthetic bacterium Rhodobacter sphaeroides R-26.1. While the antenna containing species showed large and positive variable fluorescence (Fv) relative to the constant (initial) fluorescence (F0) (Fv/F0 ∼ 4.5 in whole cell), the isolated RC had smaller and negative change (Fv/F0 ∼ -0.6). The variable fluorescence of the cells increased steadily in the function of the age of the cultivated bacterium: Fv/F0 ∼ 2 for young cells and Fv/F0 ∼ 4.5 for old cells while the rise time of the fluorescence induction remained constant (∼ 2 ms). In chromatophore, 7 times higher rate was measured than in isolated reaction center under identical experimental conditions. The results obtained under different conditions are interpreted by an extended version of the Lavergne-Trissl model where the simultaneously measured fluorescence inductions from the antenna and the RC can be separately expressed.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalActa Biologica Szegediensis
Volume51
Issue number1
Publication statusPublished - Dec 1 2007

Keywords

  • Bacteriochlorophyll
  • Chromatophore
  • Fluorescence
  • Harvesting the sun
  • Photosynthesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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