Fluorescence assay to predict activity of the glycopeptide antibiotics

Vladimir Vimberg, Radek Gazak, Zsolt Szűcs, Aniko Borbás, Pal Herczegh, Jorunn Pauline Cavanagh, Leona Zieglerova, Jan Závora, Václava Adámková, Gabriela Balikova Novotna

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Here, we describe a fluorescent assay developed to study competitive binding of the glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide antibiotics strongly depends on the hydrophobicity of the substitutes, with the best antibacterial activity of the glycopeptide antibiotics equally sharing properties of binding to d-Ala–d-Ala residues of the nascent peptidoglycan and to the membrane.

Original languageEnglish
Pages (from-to)114-117
Number of pages4
JournalJournal of Antibiotics
Volume72
Issue number2
DOIs
Publication statusPublished - Feb 1 2019

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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  • Cite this

    Vimberg, V., Gazak, R., Szűcs, Z., Borbás, A., Herczegh, P., Cavanagh, J. P., Zieglerova, L., Závora, J., Adámková, V., & Balikova Novotna, G. (2019). Fluorescence assay to predict activity of the glycopeptide antibiotics. Journal of Antibiotics, 72(2), 114-117. https://doi.org/10.1038/s41429-018-0120-5