Fluorescence assay to predict activity of the glycopeptide antibiotics

Vladimir Vimberg, Radek Gazak, Zsolt Szűcs, A. Borbás, P. Herczegh, Jorunn Pauline Cavanagh, Leona Zieglerova, Jan Závora, Václava Adámková, Gabriela Balikova Novotna

Research output: Contribution to journalArticle

Abstract

Here, we describe a fluorescent assay developed to study competitive binding of the glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide antibiotics strongly depends on the hydrophobicity of the substitutes, with the best antibacterial activity of the glycopeptide antibiotics equally sharing properties of binding to d-Ala–d-Ala residues of the nascent peptidoglycan and to the membrane.

Original languageEnglish
JournalJournal of Antibiotics
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Glycopeptides
Fluorescence
Anti-Bacterial Agents
Competitive Binding
Peptidoglycan
Hydrophobic and Hydrophilic Interactions
Bacteria
Membranes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Fluorescence assay to predict activity of the glycopeptide antibiotics. / Vimberg, Vladimir; Gazak, Radek; Szűcs, Zsolt; Borbás, A.; Herczegh, P.; Cavanagh, Jorunn Pauline; Zieglerova, Leona; Závora, Jan; Adámková, Václava; Balikova Novotna, Gabriela.

In: Journal of Antibiotics, 01.01.2018.

Research output: Contribution to journalArticle

Vimberg, V, Gazak, R, Szűcs, Z, Borbás, A, Herczegh, P, Cavanagh, JP, Zieglerova, L, Závora, J, Adámková, V & Balikova Novotna, G 2018, 'Fluorescence assay to predict activity of the glycopeptide antibiotics', Journal of Antibiotics. https://doi.org/10.1038/s41429-018-0120-5
Vimberg, Vladimir ; Gazak, Radek ; Szűcs, Zsolt ; Borbás, A. ; Herczegh, P. ; Cavanagh, Jorunn Pauline ; Zieglerova, Leona ; Závora, Jan ; Adámková, Václava ; Balikova Novotna, Gabriela. / Fluorescence assay to predict activity of the glycopeptide antibiotics. In: Journal of Antibiotics. 2018.
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AU - Szűcs, Zsolt

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AU - Cavanagh, Jorunn Pauline

AU - Zieglerova, Leona

AU - Závora, Jan

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