Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis: DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production

Takashi Sugita, Kanji Takeo, Misako Ohkusu, Eric Virtudazo, Masako Takashima, Emi Asako, Fumiko Ohshima, Satoshi Harada, Chikako Yanaka, Akemi Nishikawa, Laszlo Majoros, M. Sipiczki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The opportunistic fungal pathogens Candida inconspicua and C. norvegensis are very rarely isolated from patients and are resistant to fluconazole. We collected 38 strains of the two microorganisms isolated from Europe and Japan, and compared the polymorphism of the rRNA intergenic spacer (IGS) and internal transcribed spacer (ITS) regions, antifungal drug susceptibility, and extracellular enzyme production as a potential virulence factor. While the IGS sequences of C. norvegensis were not very divergent (more than 96.7% sequence similarity among the strains), those of C. inconspicua showed remarkable diversity, and were divided into four genotypes with three subtypes. In the ITS region, no variation was found in either species. Since the sequence similarity of the two species is approximately 70% at the ITS region, they are closely related phylogenetically. Fluconazole resistance was reconfirmed for the two microorganisms but they were susceptible to micafungin and amphotericin B. No strain of either species secreted aspartyl proteinase or phospholipase B. These results provide basal information for accurate identification, which is of benefit to global molecular epidemiological studies and facilitates our understanding of the medical mycological characteristics of C. inconspicua and C. norvegensis.

Original languageEnglish
Pages (from-to)761-766
Number of pages6
JournalMicrobiology and Immunology
Volume48
Issue number10
Publication statusPublished - 2004

Fingerprint

Intergenic DNA
yeast proteinase B
Fluconazole
Candida
Lysophospholipase
Aspartic Acid Proteases
Amphotericin B
Virulence Factors
Enzymes
Pharmaceutical Preparations
Epidemiologic Studies
Japan
Genotype
micafungin

Keywords

  • Candida inconspicua
  • Candida norvegensis
  • Drug susceptibility
  • Intergenic spacer region

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis : DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production. / Sugita, Takashi; Takeo, Kanji; Ohkusu, Misako; Virtudazo, Eric; Takashima, Masako; Asako, Emi; Ohshima, Fumiko; Harada, Satoshi; Yanaka, Chikako; Nishikawa, Akemi; Majoros, Laszlo; Sipiczki, M.

In: Microbiology and Immunology, Vol. 48, No. 10, 2004, p. 761-766.

Research output: Contribution to journalArticle

Sugita, T, Takeo, K, Ohkusu, M, Virtudazo, E, Takashima, M, Asako, E, Ohshima, F, Harada, S, Yanaka, C, Nishikawa, A, Majoros, L & Sipiczki, M 2004, 'Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis: DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production', Microbiology and Immunology, vol. 48, no. 10, pp. 761-766.
Sugita, Takashi ; Takeo, Kanji ; Ohkusu, Misako ; Virtudazo, Eric ; Takashima, Masako ; Asako, Emi ; Ohshima, Fumiko ; Harada, Satoshi ; Yanaka, Chikako ; Nishikawa, Akemi ; Majoros, Laszlo ; Sipiczki, M. / Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis : DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production. In: Microbiology and Immunology. 2004 ; Vol. 48, No. 10. pp. 761-766.
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