Flowing eluent wall processes in OPLC: Using segmentation of non-segmented adsorbent layer for single and parallel separations

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Abstract

A new concept, the flowing eluent walls (FEW) process, for segmentation of a non-segmented adsorbent bed, has been used for single-and multi-channel on-line overpressured-layer chromatography (OPLC). The FEW process leads to active and non-active regions on the adsorbent layer during the separation process. Mobile phase only is introduced to the non-active part of the layer whereas mobile phase and sample can be admitted to the active part, thus the non-homogeneous part of the adsorbent bed can be excluded from the separation process. The FEW configurations at the inlet side (FEW-I) eliminate the edge effect of OPLC in single-sample injections and the effect of sample-mixing between neighboring lanes in multi-channel separations. The FEW arrangements at the inlet and outlet sides (FEW-I/O) enable separate detection and collection of the components of the separation, and FEW lines solve the problems of joint detection of sample components and contaminants originating from the sealing material. One-channel OPLC with the FEW configuration is suitable for rapid isolation in different preparative ranges. Analytical and preparative TLC plates (20 cm × 20 cm) can be loaded with 5-25 mg and 25-125 mg, respectively. The multi-channel solution will be a tool for high-throughput analysis using efficient fine, superfine, or monolithic layers. The four and eight-channel versions can be used for high-throughput parallel analysis and for micro-preparative parallel isolation. The FEW concept provides the possibility of real multi-channel liquid chromatographic separation on a non-segmented layer and column shaped adsorbent bed.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalJournal of Planar Chromatography - Modern TLC
Volume17
Issue number6
Publication statusPublished - Nov 2004

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Chromatography
Adsorbents
Throughput
Joints
Sealants
Injections
Impurities
Liquids

Keywords

  • FEW-OPLC
  • Flowing eluent walls, FEW
  • High-throughput
  • Optimum performance laminar chromatography, OPLC
  • Overpressured-layer chromatography, OPLC
  • Parallel OPLC isolation
  • Parallel OPLC separations
  • Single- and multi-channel OPLC

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Flowing eluent wall processes in OPLC: Using segmentation of non-segmented adsorbent layer for single and parallel separations",
abstract = "A new concept, the flowing eluent walls (FEW) process, for segmentation of a non-segmented adsorbent bed, has been used for single-and multi-channel on-line overpressured-layer chromatography (OPLC). The FEW process leads to active and non-active regions on the adsorbent layer during the separation process. Mobile phase only is introduced to the non-active part of the layer whereas mobile phase and sample can be admitted to the active part, thus the non-homogeneous part of the adsorbent bed can be excluded from the separation process. The FEW configurations at the inlet side (FEW-I) eliminate the edge effect of OPLC in single-sample injections and the effect of sample-mixing between neighboring lanes in multi-channel separations. The FEW arrangements at the inlet and outlet sides (FEW-I/O) enable separate detection and collection of the components of the separation, and FEW lines solve the problems of joint detection of sample components and contaminants originating from the sealing material. One-channel OPLC with the FEW configuration is suitable for rapid isolation in different preparative ranges. Analytical and preparative TLC plates (20 cm × 20 cm) can be loaded with 5-25 mg and 25-125 mg, respectively. The multi-channel solution will be a tool for high-throughput analysis using efficient fine, superfine, or monolithic layers. The four and eight-channel versions can be used for high-throughput parallel analysis and for micro-preparative parallel isolation. The FEW concept provides the possibility of real multi-channel liquid chromatographic separation on a non-segmented layer and column shaped adsorbent bed.",
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