Flow-Reversal Experiments with Macromolecules to Measure Column End Efficiency and Bed Heterogeneity

Dóra Zelenyánszki, Adrienn Mester, Attila Felinger

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A flow-reversal method combined with peak parking has been introduced recently to determine the band broadening occurring at the two respective column ends and in the bed of the packing material. Flow-reversal has a peak compression effect, therefore, the peaks observed are always narrower and more symmetrical than the peaks obtained without reversing the flow. This phenomenon can originate from the compensation of the multipath dispersion effects. In the present study, peak parking and flow-reversal measurements were extended to macromolecules and carried out with human insulin. We observed that the peaks of insulin are always narrower with reversed flow than without reversing the flow, and the compression effect can be significantly larger than it is for small molecules. The contributions of the column inlet and outlet to the total band variance have been characterized.

Original languageEnglish
Pages (from-to)1303-1309
Number of pages7
JournalChromatographia
Volume82
Issue number9
DOIs
Publication statusPublished - Sep 1 2019

Keywords

  • Arrested flow
  • Axial heterogeneity
  • Column end structure
  • Flow-reversal
  • Peak parking

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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